Cellular Processing of the Large Glycoprotein of Lacrosse Virus (Family Bunyaviridae); Implications for Virion Assembly and Host Defense
LaCrosse virus (LAC) is a major causative agent of mosquito-borne human encephalitis in the USA (1). LAC is an enveloped RNA virus containing two glycoproteins, Gl and G2 (2). Based on electron micrographs of LAC-infected cells, it is thought that LAC derives its lipid envelope by budding from host smooth membranes rather than from the plasma membrane (3). We have demonstrated that the LAC Gl protein accumulates in a juxtanuclear region (probably Golgi) and not at the plasma membrane of the infected cell. Further evidence suggests that Gl is inefficiently modified in Golgi (4).
KeywordsSaccharide EDTA Methionine Oligosaccharide Encephalitis
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- 1.Bishop, D.H.L. and Shope, R.E. (1979). Bunyaviridae. In: Comprehensive Virology, Vol. 14. H. Fraenkel-Conrat and R.R. Wagner, Eds. Plenum Press, New York, Chapter 1, pp. 1–156.Google Scholar
- 6.Vitranen, I., Ekblom, P. and Laurila, P. (1980). Subcellular compartmentalization of saccharide moieties in cultured normal and malignant cells. J. Cell Biol. 85:249–434.Google Scholar