Electron Microscopic Examination of the Inflammatory Response of Guinea Pig Neutrophils and Macrophages to Legionella Pneumophila

  • Sheila Moriber Katz
  • Shahab Hashemi
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 162)


We studied the ultrastructural morphology of neutrophils and macrophages from splenic and pulmonary lesions of guinea pigs inoculated with Legionella pneumophila. Both organs exhibited a similar mixture of these two inflammatory cells. We used 10 male Hartley guinea pigs. Four were infected intranasally with 0.5 ml of inoculum containing 5×10 organisms. Four were infected intra-peritoneally with 0.5 ml of inoculum containing 5×106 organisms. The remaining two were inoculated intranasally with 0.5 ml of sterile diluent. All animals were autopsied three days after inoculation, and the pulmonary and splenic tissues were prepared for electron microscopic studies.


Rough Endoplasmic Reticulum Electron Microscopic Examination Intracellular Parasite Splenic Tissue Plasmic Reticulum 
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  1. 1.
    Horwitz, M.A. and Silverstein, S.C. (1981). Interaction of the Legionnaires’ disease bacterium (Legionella pneumophila) with human phagocytes. J. Exp. Med., 153:386–397.PubMedCrossRefGoogle Scholar
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    Feeley, J.C., Gorman, G.W. and Gibson, R.J. (1979). Primary isolation media and methods. In: “Legionnaires” the disease, the bacterium, the methodology. Ed: Jones, G.L. and Hebert, G.A., U.S. Dept. of Health, Education and Welfare.Google Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • Sheila Moriber Katz
    • 1
  • Shahab Hashemi
    • 1
  1. 1.Department of PathologyHahnemann Medical College and HospitalPhiladelphiaUSA

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