Monoclonal Antibodies to Salmonella Typhimurium and Escherichia Coli Lipopolysaccharides
Monoclonal antibodies reacting to lipopolysaccharides (LPS) of Salmonella typhimurium and a slow-lactose-fermenting strain of Escherichia coli (0 serotype 81) were made by immunization of BALB/c mice with trichloroacetic acid-extracted LPS and fusion of lymphoid cells with Sp2/0-Agl4 cells. Fusion supernatants were screened by radioimmunoassay against phenol/water-extracted LPS. Some of the antibodies reacted with LPS from one bacterial species but not the other, suggesting that the antibodies are directed against the 0-specific side chains of the LPS. The fine specificity of one of the antibodies was studied by inhibition of radio-immunoassay by monosaccharides. Rhamnose and mannose inhibited best, followed by galactose and glucose, while the weakest inhibition was given by N-acetylglucosamine, which is not part of the S. typhimurium 0 antigen. Agglutination and immunofluorescence results suggest that the determinant is on the bacterial surface. Two other hybridomas were tested by Alf Lindberg and Stefan Svenson in an enzyme-linked immunosorbent assay against LPS and oligosaccharides. One antibody is directed against an α-galactose (1→2) mannose disaccharide, while the other appears to recognize factor 5 (2-acetyl-abequose).
KeywordsMesenteric Lymph Node Precursor Frequency Mesenteric Lymph Node Cell Intact Bacterial Cell Salmonella TYPHIMURIUM
Unable to display preview. Download preview PDF.
- 3.Cebra, J.J, Crandall, C.A., Gearhart, P.J., Robertson, S.M., Tseng, J. and Watson, P.M. (1979). In: Immunology of Breast Milk (P. L. Ogra and D. H. Dayton, eds.) pp. 1–16, Raven Press, New York.Google Scholar
- 6.Klinman, N.R., Pickard, A.R., Sigal, N.H., Gearhart, P.J., Metcalf, E.S. and Pierce, S.K. (1976). Ann. Immunol. (Inst. Pasteur) 1270:489–502.Google Scholar
- 8.Lefkovits, I. and Pernis, B. (1979). Immunological Methods, Academic Press, New York, p. 364.Google Scholar
- 9.McCabe, W.R. (1980). Seminars Infec. Dis. III 38–88.Google Scholar
- 11.Mosier, D.E., Mond, J.J., Zitron, I., Scher, I. and Paul, W.E. (1977). pp. 699–706 In: Immune System: Genetics and Regulation. (E.E. Sercarz, L.A. Herzenberg and C.F. Fox, eds.) Academic Press, New York.Google Scholar