Monoclonal Antibodies to Salmonella Typhimurium and Escherichia Coli Lipopolysaccharides

  • Jack L. Komisar
  • John J. Cebra
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 162)


Monoclonal antibodies reacting to lipopolysaccharides (LPS) of Salmonella typhimurium and a slow-lactose-fermenting strain of Escherichia coli (0 serotype 81) were made by immunization of BALB/c mice with trichloroacetic acid-extracted LPS and fusion of lymphoid cells with Sp2/0-Agl4 cells. Fusion supernatants were screened by radioimmunoassay against phenol/water-extracted LPS. Some of the antibodies reacted with LPS from one bacterial species but not the other, suggesting that the antibodies are directed against the 0-specific side chains of the LPS. The fine specificity of one of the antibodies was studied by inhibition of radio-immunoassay by monosaccharides. Rhamnose and mannose inhibited best, followed by galactose and glucose, while the weakest inhibition was given by N-acetylglucosamine, which is not part of the S. typhimurium 0 antigen. Agglutination and immunofluorescence results suggest that the determinant is on the bacterial surface. Two other hybridomas were tested by Alf Lindberg and Stefan Svenson in an enzyme-linked immunosorbent assay against LPS and oligosaccharides. One antibody is directed against an α-galactose (1→2) mannose disaccharide, while the other appears to recognize factor 5 (2-acetyl-abequose).


Mesenteric Lymph Node Precursor Frequency Mesenteric Lymph Node Cell Intact Bacterial Cell Salmonella TYPHIMURIUM 
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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • Jack L. Komisar
    • 1
  • John J. Cebra
    • 1
  1. 1.Department of BiologyUniversity of PennsylvaniaPhiladelphiaUSA

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