Macrophage Oxygen-Dependent Killing of Intracellular Parasites: Toxoplasma and Leishmania
The importance of cell-mediated immunity in host resistance to infection caused by intracellular pathogens has been well-established. Similarly, it has been known for some time that monocytes and macrophages play primary roles in the efferent limb of the cellular response to such infections (1–9). The mechanisms by which these phagocytes exert antimicrobial activity, however, have been largely unexplored. During the past several years, work in this and other laboratories has indicated that the generation of toxic oxygen intermediates contributes importantly to the ability of mononuclear phagocytes to kill or inhibit the growth of ingested microbial pathogens (10–20). Thus, considerable evidence is now available to suggest that the well-recognized oxygen-dependent mechanism present in polymorphonuclear leukocytes, which generates a number of toxic products including superoxide anion (O 2 - ), hydrogen peroxide (H2O2), and hydroxyl radical (OH) (21–23), is also present and operative in monocytes and macrophages.
KeywordsPhorbol Myristate Acetate Oxidative Burst Respiratory Burst Activity Proteose Peptone Antiprotozoal Activity
Unable to display preview. Download preview PDF.
- 13.Murray, H.W., Nathan, C.F. and Cohn, Z.A. (1980). J. Exp. Med. 152:1601.Google Scholar
- 20.Klebanoff, S.J. and Hamon, C.B. (1975). In Mononuclear Phagocytes in Immunity, Infection, and Pathology, Blackwell Scientific Publications, Oxford, p. 507.Google Scholar
- 30.Murray, H.W. (1981). Manuscript submitted.Google Scholar
- 31.Baehner, R.L., Johnston, R.B. and Nathan, D.G. (1972). J. Reticulendothel Soc. 12:150.Google Scholar