Guinea Pig Alveolar Macrophages Probably Kill M. Tuberculosis H37Rv and H37Ra In Vivo by Producing Hydrogen Peroxide

  • P. S. Jackett
  • P. W. Andrew
  • V. R. Aber
  • D. B. Lowrie
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 162)


The major phagocytic cell involved in host resistance to Mycobacterium tuberculosis infection is probably the alveolar macrophage. It seems likely that macrophages, at least in the immune animal, can kill M. tuberculosis (1) and this might involve the production of hydrogen peroxide (H2O2) by the macrophages. Macrophages release H2O2 in vitro when exposed to phagocytic stimuli or certain soluble agents that perturb the plasma membrane (8, 9). H2O2 can kill M. tuberculosis and resistance to H2O2 in vitro correlates with high virulence in the guinea pig (11, 5).


Alveolar Macrophage Tuberculosis H37Rv Mycobacterium Tuberculosis Infection H202 Release Royal Postgraduate Medical School 


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  1. 1.
    Greening, A.P., Rees, A.D.M. and Lowrie, D.B. (1980). Human alveolar macrophage staphylocidal function. Clin. Sci. 59, 14.Google Scholar
  2. 2.
    Guilbault, G.G., Brignac, P.J. and Juneau, M. (1968). New substrates for fluorimetric determination of oxidative enzymes. Anal. Chem. 40, 1256–1263.PubMedCrossRefGoogle Scholar
  3. 3.
    Harmsen, A.G. and Jeska, E.L. (1980). Surface receptors on porcine alveolar macrophages and their role in phagocytosis. J. Reticuloendoth. Soc. 27, 631–637.Google Scholar
  4. 4.
    Hunninghake, G.W. and Fauci, A.S. (1976). Immunological reactivity of the lung. 1. A guinea-pig model for the study of pulmonary mononuclear cell subpopulations. Cell. Immunol. 26, 89–97.PubMedCrossRefGoogle Scholar
  5. 5.
    Jackett, P.S., Aber, V.R. and Lowrie, D.B. (1978). Virulence and resistance to superoxide, low pH and hydrogen peroxide among strains of Mycobacterium tuberculosis. J. Gen. Microbiol, 104, 37–45.PubMedGoogle Scholar
  6. 6.
    Jackett, P.S., Aber, V.R., Mitchison, D.A. and Lowrie, D.B. (1981). The contribution of hydrogen peroxide resistance to virulence of Mycobacterium tuberculosis during the first six days after intravenous infection of normal and BCG-vaccinated guinea-pigs. Br. J. Exp. Path. 62, 34–40.Google Scholar
  7. 7.
    Johnston, R.B., Godzik, C.A. and Cohn, Z.A. (1978). Increased superoxide anion production by immunologically activated and chemically elicited macrophages. J. Exp. Med., 148, 115–127.PubMedCrossRefGoogle Scholar
  8. 8.
    Kaneda, M., Kakinum, K., Yamaguchi, T. and Shimada, K. (1980). Comparative studies on alveolar macrophages and polymorphonuclear leukocytes. II. The ability of guinea-pig alveolar macrophages to produce H2O2. J. Biochem., 88, 1159–1165.PubMedGoogle Scholar
  9. 9.
    Klebanoff, S.J. and Hamon, C.B. (1975). Antimicrobial systems of mononuclear phagocytes. In Mononuclear phagocytes in immunity, infection and pathology. pp. 507–529. Edited by R. van Furth. Oxford: Blackwell Scientific.Google Scholar
  10. 10.
    Lurie, M.B. (1964). Resistance to tuberculosis; experimental studies in native and acquired defensive mechanisms. Cambridge, Mass: Harvard University Press.Google Scholar
  11. 11.
    Mitchison, D.A., Selkon, J.B. and Lloyd, J. (1963). Virulence in the guinea-pig, susceptibility to hydrogen peroxide, and catalase activity of isoniazid-sensitive tubercle bacilli from South Indian and British patients. J. Path. Bact. 86, 377–386.PubMedCrossRefGoogle Scholar
  12. 12.
    Montarroso, A.M. and Myrvik, Q.N. (1978). The effect of BCG vaccination on the IgG and complement receptors on rabbit alveolar macrophages. J. Reticuloendoth. Soc., 24, 93–99.Google Scholar
  13. 13.
    Nathan, C.F., Nogueira, N., Juangbhanich, C.W., Ellis, J. and Cohn, Z.A. (1979). Activation of macrophages in vivo and in vitro. Correlation between hydrogen peroxide release and killing of Trypanosoma cruzi. J. Exp. Med., 149, 1056–1068.PubMedCrossRefGoogle Scholar
  14. 14.
    Wolff, L.J., Boxer, L.A., Allen, J.M. and Baehner, R.L. (1978). The selective effect of hypoxia on the guinea-pig alveolar macrophage membrane. J. Reticuloendoth. Soc., 24, 377–382.Google Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • P. S. Jackett
    • 1
  • P. W. Andrew
    • 1
  • V. R. Aber
    • 1
  • D. B. Lowrie
    • 1
  1. 1.MRC Unit for Laboratory Studies of TuberculosisRoyal Postgraduate Medical SchoolLondonEngland

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