Development and Regulation of Cardiac Muscarinic Acetylcholine Receptor Number, Function and Gyanyl Nucleotide Sensitivity

  • Stan W. Halvorsen
  • Bronia Engel
  • Dale D. Hunter
  • Neil M. Nathanson
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 161)


Acetylcholine is released from parasympathetic nerve terminals and binds to muscarinic acetylcholine (mACh) receptors to elicit negative chronotropic and inotropic effects on the heart. The molecular mechanisms for these cardiac effects are not well understood. Investigation of the mACh receptor has been greatly facilitated by the development of the potent cholinergic antagonist [3H] quinuclidinyl benzilate (QNB) which binds specifically and with high affinity to the mACh receptor (1). [3H]QNB can be used to study the interaction of agonists and antagonists with the receptor and quantitate the number of receptors present in cells and tissues.


Adenylate Cyclase Muscarinic Receptor Adenylate Cyclase Activity Receptor Number Muscarinic Acetylcholine Receptor 


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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • Stan W. Halvorsen
    • 1
  • Bronia Engel
    • 1
  • Dale D. Hunter
    • 1
  • Neil M. Nathanson
    • 1
  1. 1.Department of PharmacologyUniversity of WashingtonSeattleUSA

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