Ion Movements in Adult Rat Heart Myocytes
Recent improvements in methodology for the isolation of adult heart cells (6, 9, 10, 18, 22, 25, 31, 36, 46) yield myocytes that are more suitable for the study of cellular and intracellular ion movements than those previously available. For example, 92% of the cells in the myocyte preparations currently in use in our laboratory are viable by trypan blue exclusion, 85% show the elongated, rod-like configuration of heart cells in situ (Fig. 1A), and the rod cells show morphology in the electron microscope that is indistinguishable from cells in intact heart tissue (Fig. 2). These cells also contain enzyme profiles corresponding to those of heart cells in situ (34) and ATP, total adenine nucleotide, and creatine phosphate at levels quite comparable to those found in intact heart tissue (19,24, and 34 nmol • mg protein-1, respectively; Ref. 22).
KeywordsHeart Cell Anaerobic Incubation Total Adenine Nucleotide Heart Myocytes Adenine Nucleotide Pool
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