Studies of Substrate Metabolism in Isolated Myocytes

  • John J. Spitzer
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 161)


Although techniques of isolating heart myocytes from adult animals have heen available for over a decade, the often encountered low yield, poor viability, contamination by non-muscle cells and extreme sensitivity to the presence of calcium ions have limited the use of such preparations. During the last two to three years several laboratories have reported techniques of isolating adult myocardial cells with good yield, good viability, minimal contamination by non-muscle cells, as well as calcium-tolerance (1,2,3,4). Thus, the use of freshly isolated adult heart myocytes in myocardial biology has become more widespread (5) primarily, because of the following advantages of the preparation: a) Homogeneous population of myocytes is obtained which is virtually free of other cellular elements, whereas in the rat heart, myocytes comprise only about 25 percent of the total cell population (6). b) A cell population, free of neuronal and hormonal influences is obtained. c) During an experiment the isolated myocyte preparation can be divided into several aliquots and thus, internal controls for each preparation can be employed. d) Cells can be exposed to environmental changes without the necessity to consider capillary and extracellular barriers.


Substrate Metabolism Atrial Natriuretic Factor Perfuse Heart Atrial Myocytes Urinary Sodium Excretion 


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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • John J. Spitzer
    • 1
  1. 1.Department of PhysiologyLouisiana State University Medical CenterNew OrleansUSA

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