T-Cell Growth Factor, a Lymphocytotrophic Hormone

  • Kendall A. Smith
Part of the Nobel Foundation Symposia Published by Plenum book series (NOFS, volume 55)


Despite an intensive effort over the past twenty years, our understanding of the process by which the immune response is regulated remains rudimentary. In part, our difficulties in this area have related to the morphological homogeneity but functional heterogeneity of the cells responsible for the immune reaction. The dissection of the mechanisms that underlie the regulatory control of the immune response has been facilitated, however, by the development of cell culture systems that can be made to generate measurable immune reactions, and thus mimic immune responses as they are thought to occur in vivo. As a result, it has become apparent that the differentiation of lymphocytes to become functionally competent immunocytes, is dependent upon a complex intercellular communication system that involves not only different classes and subclasses of lymphocytes, but macrophages as well. Consequently, considerable attention has focused on the roles played by each cell type, and the mechanisms operative.


Human Peripheral Blood Lymphocyte Polypeptide Hormone Isolate Plasma Membrane Purify Plasma Membrane Secondary Mediator 
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  1. 1.
    P. B. Medawar, in: “Immunopotentiation,” Associated Scientific Publishers, Amsterdam p. 343 (1973).Google Scholar
  2. 2.
    D. A. Morgan, F. W. Ruscetti, and R. Gallo, Selective in vitro growth of T-lymphocytes from normal human bone marrows, Science (Wash. D.C.) 193: 1007 (1976).CrossRefGoogle Scholar
  3. 3.
    E-L. Larsson, A. Coutinho and C. Martinez-A, A suggested mecha- nism for T lymphocyte activation: Implications on the acquisition of functional reactivities, Immunol. Rev. 51: 61 (1980).PubMedCrossRefGoogle Scholar
  4. 4.
    F. W. Ruscetti, and R. C. Gallo, Human T-lymphocyte growth factor: regulation of growth and function of T lymphocytes, Blood 57: 379 (1981).PubMedGoogle Scholar
  5. 5.
    K. A. Smith, and F. W. Ruscetti, T-cell growth factor and the culture of cloned functional T-cells, Adv. Immunol 31: 137 (1981).Google Scholar
  6. 6.
    S. Gillis, K. A. Smith, and J. D. Watson, Biochemical and biological characterization of lymphocyte regulatory molecules. II. Purification of a class of rat and human lymphokines, J. Immunol, 124: 1954 (1980).PubMedGoogle Scholar
  7. 7.
    J. W. Mier, and R. C. Gallo, Purification and some characteristics of human T-cell growth factor from phytohemagglutininstimulated lymphocyte-conditioned media, 77: 6134 (1980).Google Scholar
  8. 8.
    R. J. Robb, and K. A. Smith, Heterogeneity of human T-cell growth factor(s) due to variable glycosylation, Mol. Immunol. 18: 1087 (1981).Google Scholar
  9. 9.
    K. A. Smith, M. F. Favata, and R. Ffanza, Characteristics of human T-cell growth factor isolated by a monoclonal antibody affinity column. In preparation.Google Scholar
  10. 10.
    K. A. Smith, P. E. Baker, S. Gillis, and F. W. Ruscetti, Functional and molecular characteristics of T-cell growth factor, Mol. Immunol 17: 579 (1980).Google Scholar
  11. 11.
    K. A. Smith, S. Gillis, P. E. Baker, D. McKenzie, and F. W. Ruscetti, T-cell growth factor mediated T-cell proliferation, Ann. N. Y. Acad Sci. 332–423 (1979).Google Scholar
  12. 12.
    A. Coutinho, E. L. Larsson, K-O Gronvik, and J. Anderson, Studies on T-lymphocyte activation. II. The target cells for concanavalin-A-induced growth factors, Eur. J. Immunol 9: 587 (1979)PubMedCrossRefGoogle Scholar
  13. 13.
    G. D. Bonnard, D. Yasaka, and D. Jacobson, Ligand-activated T-cell growth factor-induced proliferation: Absorption of T-cell growth factor by activated T-cells, J. Immunol, 123: 2704 (1979).PubMedGoogle Scholar
  14. 14.
    R. J. Robb, A. Munck, and K. A. Smith, T-cell growth factor receptors: quantitation, specificity, and biological relevance, J. Exp. Med. 154: 1455 (1981).PubMedCrossRefGoogle Scholar
  15. 15.
    D. E. Cummings and K. A. Smith, Characteristics of radiolabeled T-cell growth factor binding to isolated plasma membranes. In preparation.Google Scholar
  16. 16.
    F. W. Ruscetti, D. A. Morgan, and R. C. Gallo, Functional and morphologic characterization of human T-cells continuously grown in vitro, J. Immunol, 119: 131 (1977).PubMedGoogle Scholar
  17. 17.
    E. L. Larsson, and A. Coutinho, On the role of mitogenic lectins in T-cell triggering, Nature (Land.), 280: 239 (1979).CrossRefGoogle Scholar
  18. 18.
    S. Strickland, and J. N. Loeb, Obligatory separation of hormone binding and biological response curves in systems dependent upon secondary mediators of hormone action, Proc. Nat. Acad. Sci. USA, 79: 1366 (1981).CrossRefGoogle Scholar
  19. 19.
    K. A. Smith, Continuous cytotoxic T-cell lines, in: “Contemporary topics in immunobiology,” N. L. Warner, ed., Plenum Publishing Corporation, New York (1980).Google Scholar
  20. 20.
    M. Krupp, and M. D. Lane, On the mechanism of ligand-induced down-regulation of insulin receptor levels in the liver cell, J. Biol. Chem. 256: 1689 (1981).PubMedGoogle Scholar
  21. 21.
    P. J. Gorden, L. Carpentier, P. Freychet, and L. Orci, Internalization of polypeptide hormones. Mechanism, intracellular location, and significance. Diabetolgia 18: 263 (1980).CrossRefGoogle Scholar
  22. 22.
    A. C. King, and P. Cuatrecasas, Peptide hormone-induced receptor mobility, aggregation, and internalization, New Eng. J. Med. 305: 77 (1981).CrossRefGoogle Scholar
  23. 23.
    J. Kumagai, H. Akiyama, S. Iwashita, H. Lida, and I. Yahara, In vitro regeneration of resting lymphocytes from stimulated lymphocytes and its inhibition by insulin, J. Immunol. 126: 1249 (1981).PubMedGoogle Scholar
  24. 24.
    T. B. Strom, and J. D. Bangs, Human serum-free mixed lymphocyte response: the stereospecific effect of insulin and its potentiation by transferin, 128: 1555, J. Immunol. (1982).PubMedGoogle Scholar
  25. 25.
    S. Gillis, M. Ferm, W. Ou, and K. A. Smith, T-cell growth factor: parameters of production and a quantitative micro-assay for activity, J. Immunol, 120: 2027 (1978).PubMedGoogle Scholar
  26. 26.
    M. H. Schreier, N. N. Iscove, R. Tees, L. Aarden, and H. von Boehmer, Clones of killer and helper T-cells: growth requirements, specificity and retention of function in longterm culture, Immunol. Rev. 51: 315, (1980).PubMedCrossRefGoogle Scholar
  27. 27.
    H. Northoff, C. Carter, and J. J. Oppenheim, Inhibition of concanavalin A-induced human lymphocyte mitogenic factor (interleukin-2) production by suppressor T lymphocytes, J. Immunol. 125: 1823, (1980).PubMedGoogle Scholar
  28. 28.
    T. F. Dougherty, and A. White, Influence of hormones on lymphoid tissue structure and function. The role of the pituitary adrenotrophic hormone in the regulation of the lymphocytes and other cellular elements of the blood, Endocrinology, 35: 1 (1944).CrossRefGoogle Scholar
  29. 29.
    M. Ishidate, Jr., and D. Metcalf, The pattern of lymphophoiesis in the mouse thymus after cortisone administration or adrenalectomy, Austral. J. Exp. Biol, 41: 637 (1963).CrossRefGoogle Scholar
  30. 30.
    J. B. Murphy, and Ernest Sturm, The lymphoid tissue and antibody formation, Proc. Soc. Exp. Biol. 66: 303, (1947).PubMedGoogle Scholar
  31. 31.
    R. J. Graff, M. A. Lappe, and G. D. Snell, The influence of the gonads and adrenal glands on the immune response to skin grafts, Transplantation, 7: 105, (1969).PubMedCrossRefGoogle Scholar
  32. 32.
    J. E. Castro, D. N. H. Hamilton, Adrenalectomy and orchidectomy as immunopotentiating procedures, Transplantation, 13: 614 (1972).CrossRefGoogle Scholar
  33. 33.
    C. B. Streng, and P. Nathan, The immune response in steroid deficient mice, Immunology, 24: 559 (1973).PubMedGoogle Scholar
  34. 34.
    C. R. Bishop, J. W. Athens, D. R. Boggs, H. R. Warner, G. E. Cartwright, and M. M. Wintrobe, XIII. a non-steady-state kinetic evaluation of the mechanism of cortisone-induced granulocytosis, J. Clin. Invest. 47: 249 (1968).PubMedCrossRefGoogle Scholar
  35. 35.
    J. Thompson, and R. Van Furth, The effect of glucocorticosteroids on the proliferation and kinetics of promonocytes and monocytes of the bone marrow, J. Exp. Med. 137: 10 (1973).PubMedCrossRefGoogle Scholar
  36. 36.
    H. O. Besedovsky, A. Del Rey, and E. Sorkin, Lymphokine-containing supernatants from con A-stimulated cells increase corticosterone blood levels, J. Immunol. 126: 385 (1981).PubMedGoogle Scholar
  37. 37.
    H. O. Besedovsky, E. Sorkin, M. Keller, and J. Muller, Changes in blood hormone levels during the immune response Proc. Exp. Biol. Med., 150: 466 (1975).Google Scholar
  38. 38.
    R. E. Billingham, P. L. Krohn, Effect of cortisone on survival of skin homografts in rabbits, Brit. Med. Journal, 1: 4716, 1951.Google Scholar
  39. 39.
    P. B. Medawar, and E. M. Sparrow, The effects of adrenocortical hormones, adrenocorticotrophic hormone and pregnancy on skin transplantation immunity in mice, J. Endocrinol. 14: 240 (1956).PubMedCrossRefGoogle Scholar
  40. 40.
    P. C. Nowell, Phytohemagglutinin: An initiator of mitosis in cultures of normal human leukocytes, Cancer Research 20: 462 (1960).PubMedGoogle Scholar
  41. 41.
    P. C. Nowell, Inhibition of human leukocyte mitosis by prednisone in vitro, Cancer Research, 21: 1518 (1961).PubMedGoogle Scholar
  42. 42.
    S. Gillis, G. R. Crabtree, and K. A. Smith, Glucocorticoidinduced inhibition of T-cell growth factor production. I. The effect on mitogen-induced lymphocyte proliferation J. Immunol. 123: 1624 (1979)PubMedGoogle Scholar
  43. 43.
    S. Gillis, G. R. Crabtree, and K. A. Smith, Glucocorticoidinduced inhibition of T-cell growth factor production. H. The effect on the in vitro generation of cytolytic T-cells. J. Immunol. l23: 1632 (1979).Google Scholar
  44. 44.
    K. A. Smith, T-cell growth factor and glucocorticoids: opposing regulatory hormones in neoplastic T-cell growth, Immunobiol. 161: 157 (1982).CrossRefGoogle Scholar
  45. 45.
    H. Rabin, R. F. W. Ruscetti, R. H. Neubauer, R. L. Brown, and T. G. Kawakami, Spontaneous release of a factor with T-cell growth factor activity from a continuous line of primate tumor T-cells, J. Immunol. 127: 1852, (1981).PubMedGoogle Scholar
  46. 46.
    R. G. Deeley, J. I. Gordon, A. T. H. Burns, K. P. Mullinix, M. Binkstein and R. F. Goldberger, Primary activation of the vitellogenin gene in the rooster, J. Biol. Chem. 252: 8310 (1977).PubMedGoogle Scholar
  47. 47.
    H. Aviv and P. Leder, Purification of biologically active globin messenger RNA by chromatography on oligo-thymidylic acid-cellulose, Proc. Nat. Acad. Sci. USA. 69: 1408 (1972).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • Kendall A. Smith
    • 1
  1. 1.The Department of MedicineDartmouth Medical SchoolHanoverUSA

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