Methods for Detecting and Monitoring the Resistance of Plant Pathogens to Chemicals

  • J. M. Ogawa
  • B. T. Manji
  • C. R. Heaton
  • J. Petrie
  • R. M. Sonoda


The detection and monitoring of strains of plant pathogens resistant to chemicals became of concern only recently, with the introduction of fungicides and bactericides that are highly specific, partially systemic, and very effective. Agriculturists have been fortunate in having had no resistance problems to contend with during the long period when sulfurs, copper, mercuries, dithiocarbamates, captan and similar nonsystemic fungicides and soil fumigants were in extensive use. Temporary resistance or adaptation of plant pathogens to copper was detected, but these resistant pathogens lost their resistance once they were allowed to grow on media free of the chemical (Parry and Wood, 1959). Thus, temporary adaptation of plant pathogens to some fungicides was not related to or correlated with any loss in their efficacy in controlling diseases. Decades and even centuries of repeated applications with these chemicals produced no evidence that they would fail to protect crops against loss to diseases because of the development of resistance. Concern about fungicide resistance and methods for determining resistance came with the discovery by Harding (1962) that control of Penicillium on citrus fruit was being lost through the development of resistance to ortho-phenylphenate and biphenyl.


Powdery Mildew Plant Pathogen Fusarium Oxysporum Downy Mildew Botrytis Cinerea 
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  1. Ashida, J., 1965, Adaptation of fungi to metal toxicants, Annu. Rev. Phytopathol., 3:153.CrossRefGoogle Scholar
  2. Bent, K. J., Cole, A. M., Turner, J. A. W., and Woolner, M., 1971, Resistance of cucumber mildew to dimethirimol, Proc. 6th Brit. Insecti. and Fungi. Conf., p. 274.Google Scholar
  3. Berger, R. D., 1973, Disease progress of Cercospora apii resistant to benomyl, Plant Dis. Rep., 57:837.Google Scholar
  4. Brooks, D. H., and Buckley, N. G., 1977, Results in practice 1. Cereals and grasses, in: “Systemic Fungicides,” R. W. Marsh, ed., p. 233, Butler and Tanner, Ltd.Google Scholar
  5. Chastagner, G. A., 1980, Tolerance of Botrytis tulipae to glycophene and vinclozolin, Phytopathology (in press).Google Scholar
  6. Chastagner, G. A., and Ogawa, J. M., 1979, DCNA-benomyl multiple tolerance in strains of Botrytis cinerea. Phytopathology, 69:699.CrossRefGoogle Scholar
  7. Chastagner, G. A., and Ogawa, J. M., 1979, A fungicide-wax treatment to suppress Botrytis cinerea and protect fresh-market tomatoes, Phytopathology, 56:59.CrossRefGoogle Scholar
  8. Dekker, J., and Gielink, A. J., 1979, Acquired resistance to pimari-cin in Ciadosporium cucwnerinum and Fusarium oxysporum f. sp. narcissi associated with decreased virulence, Beth. J. Pl. Pathol., 85:67.CrossRefGoogle Scholar
  9. English, A. R., and Van Helsema, G., 1954, A note on the emergence of resistant Xanthomonas and Erwinia strains by the use of streptomycin plus Terramycin combinations, Plant Dis. Rep., 38:429Google Scholar
  10. FAO Plant Production and Protection Paper on Pest Resistance to Pesticides and Crop Loss Assessment, 1979, AGP:1979/M/2. 41 p.Google Scholar
  11. Georgopoulos, S. G., and Zaracovitis, C., 1967, Tolerance of fungi to organic fungicides, Annu. Rev. Phytopathol., 5:109.CrossRefGoogle Scholar
  12. Harding, P. R., 1962, Differential sensitivity to sodium orthophenyl-phenate by biphenyl-sensitive and biphenyl-resistant strains of Prnicillium digitatum, Plant Dis. Rep., 46:100.Google Scholar
  13. Hills, F. J., and Leach, L. D., 1962, Photochemical decomposition and biological activity of p-dimethylaminobenzenediazo sodium sulfonate (Dexon), Phytophathology, 52:51.Google Scholar
  14. Holloman, D. W., 1977, Laboratory evaluation of ethirimol activity, in: “Crop Protection Agents—Their Biological Evaluation,” N. R. McFarlane, ed., pp. 505–515, Academic Press, London.Google Scholar
  15. Littrell, R. H., 1976, Techniques in monitoring for resistance in plant pathogens, Proc. Am. Phytopathol. Soc., 3:90.Google Scholar
  16. Miller, T. D., and Schroth, M. N., 1972, Monitoring the epiphytic populations of Evwinia amylovora on pear with a selective medium, Phytopathology, 62:1175.CrossRefGoogle Scholar
  17. Ogawa, J. M., Mathre, J. H., Weber, D. J., and Lyda, S. D., 1963a, Effects of 2,6-Dichloro-4-nitroaniline on Rhizopus species and its comparison with other fungicides on control of Rhizopus rot of peaches, Phytopathology, 53:950.Google Scholar
  18. Ogawa, J. M., Ramsey, R. H., and Moore, C. J., 1963b, Behavior of variants of Gilbertella persicaria arising in medium containing 2,6-dichloro-4-nitroaniline, Phytopathology, 53:97.Google Scholar
  19. Ogawa, J. M., Manji, B. T., and Bose, E., 1968, Efficacy of fungicide 1991 in reducing fruit rot of stone fruit, Plant Dis. Rep., 52:722.Google Scholar
  20. Ogawa, J. M., Gilpatrick, J. D., and Chiarappa, L., 1977, Review of plant pathogens resistant to fungicides and bactericides, FAO Plant Protection Bull., 25(3):97.Google Scholar
  21. Ogawa, J. M., Gilpatrick, J. D., Uyemoto, J. K., and Abawi, G. S., 1978a, Variations in fungal growth on various preparations of potato-dextrose agar media, Plant Dis. Rep., 62:437.Google Scholar
  22. Ogawa, J. M., Manji, B. T., Bose, E. A., Szkolnik, M., and Frate, C. A., 1978b, Methods for detection of benomyl-tolerant Moni-linia fructicola, Phytopathol. News 12(9):248 (Abstr,).Google Scholar
  23. Parry, K. E., and Wood, R. K. S., 1959, The adaptation of fungi to fungicides: Adaptation of thiram, ziram, ferbam, nabam and zineb, Ann. Appl. Biol., 47:10.CrossRefGoogle Scholar
  24. Ramsdell, D. C., and Ogawa, J. M., 1973, Systemic activity of methyl-2-benzimidazolecarbamate (MBC) in almond blossoms following prebloom sprays of benomyl MBC, Phytopathology, 63:959.CrossRefGoogle Scholar
  25. Sakurai, H., 1977, Methods of determining the drug-resistant strains in phytopathogenic bacteria and fungi and its: epidemiology in the field, Rev. J. Pestic. Sci., 2:177.CrossRefGoogle Scholar
  26. Sakurai, H., and Naito, H., 1976, A cross resistance of Pyricularia oryzae Cavara to kasugamycin and blasticidin-S, J. Antibiotics, 29:1341.CrossRefGoogle Scholar
  27. Sakurai, H., Naito, H., and Yoshida, K., 1975, Studies on cross resistance to antifungal antibiotics in kasugamycin-resistant strains of Pyricularia oryzae Cavara, Bull. Agr. Chem. Inspect. Stn. (Tokyo), 15:82.Google Scholar
  28. Schroeder, W. T., and Prowidenti, R., 1969, Resistance to benomyl in powdery mildew of cucurbits, Plant Dis. Rep.; 53:271.Google Scholar
  29. Schroth, M. N., Thompson, J. P., and Hildebrand, D. C., 1965, Isolation of Agrobacterium tumefaoiens — A. radiobaoter group from soil, Phytopathology., 55:645.Google Scholar
  30. Schroth, M. N., Beutel, J. A., Moller, W. J., and Beil, W. O., 1972, Fireblight of pears in California, current status and research progress, Calif. Plant Pathol.; 7(1):1.Google Scholar
  31. Schroth, M. N., Thomson, S. V., and Moller, W. J., 1979, Streptomycin resistance in Erwinia amylovova, Phytopathology, 69:565.CrossRefGoogle Scholar
  32. Shepherd, M. C., Bent, K. J., Woolner, M., and Cole, A. M., 1975, Proc. 8th Brit. Insecti. and Fungi. Conf.; 59.Google Scholar
  33. Sonoda, R. M., and Ogawa, J. M., 1972, Ecological factors limiting epidemics of hop downy mildew in arid climates, Rilgardia, 41:457.Google Scholar
  34. Sonoda, R. M., Ogawa, J. M., Lyons, T., and Hansen, J. A., 1970, Correlation between immobilization of zoospores by fungicides and the control of Phytophthora root and crown rot of transplanted tomatoes, Phytopathology, 60:783.CrossRefGoogle Scholar
  35. Szkolnik, M., 1978, Techniques involved in greenhouse evaluation of deciduous tree fruit fungicides, Annu. Rev. Phytopathol., 16:103.CrossRefGoogle Scholar
  36. Szkolnik, M., and Gilpatrick, J. D., 1977, Tolerance of Monilinia fructioola to benomyl in western New York State orchards, Plant Dis. Rep., 61:654.Google Scholar
  37. Tate, K. G., Ogawa, J. M., Manji, B. T., and Bose, E., 1974, Survey for benomyl-tolerant isolates of Monilinia fruotioola and Monilinia laxa in stone fruit orchards of California, Plant Dis. Rep., 58:663.Google Scholar
  38. Webster, R. K., Ogawa, J. M., and Moore, C. J., 1968, The occurrence and behavior of variants of Rhizopus stolonifer tolerant to 2,6-dichloro-4-nitroaniline, Phytopathology, 58:997.Google Scholar
  39. Whan, J. H., 1976, Tolerance of Sclerotinia fruotioola to benomyl, Plant Dis. Rep., 60:200.Google Scholar
  40. Wilson, E. E., and Ogawa, J. M., 1979, Fungal, bacterial and certain nonparasitic diseases of fruit and nut crops in California, Div. Agric. Sciences, Univ. Calif., 190 pp.Google Scholar
  41. Wolfe, M. S., and Minchin, P. M., 1976, Quantitative assessment of variations in field populations of Erysiphe graminis f. sp. hordei using mobile nurseries, Trans. Br. Mycol. Soc., 66:332.CrossRefGoogle Scholar
  42. Yaoita, T., Goh, N., Aoyagi, K., Iwano, M., and Sakurai, H., 1979, Studies on drug-resistant strain of rice blast fungus, Pyricu-laria oryzae Cavara 1. The occurrence of the multi-resistant strains of Pyricularia oryzae and its epidemiology in the field, J. Niigata Agr. Exp. Sta., No. 28 (March).Google Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • J. M. Ogawa
    • 1
  • B. T. Manji
    • 1
  • C. R. Heaton
    • 1
  • J. Petrie
    • 1
  • R. M. Sonoda
    • 1
  1. 1.Department of Plant PathologyUniversity of CaliforniaDavisUSA

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