Research on Gene Therapy

  • Martin J. Cline
Part of the Basic Life Sciences book series


I would like to begin by describing the methods that are currently available for introducing new genes into mammalian cells. These techniques have generally been applied to cells in tissue culture. Historically, the oldest method for insertion of genes into cells utilizes chromosomes as the mechanism of gene transfer. A variety of chromosomal techniques have been described [1], Recipient and donor cells can be fused together; chromosomes can be isolated and incubated with target cells for endocytosis; and finally, chromosomes can be entrapped within a nuclear membrane and the membrane can then be fused to the target cell. None of the chromosomal techniques are very efficient at gene transfer; and, in general, only about one cell in ten million is transformed. By transformed, I mean that the target cell acquires new genetic information and expresses that information for some period of time.


Bone Marrow Cell Thymidine Kinase Globin Gene Dihydrofolate Reductase Helper Virus 


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    Cline, M. J. Genetic engineering of mammalian cells: Its potential application to genetic diseases of man. J. Lab. Clin. Med. 99: 299–308 (1982).Google Scholar
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    Salser, W., B. D. Tong, H. D. Stang, J. Browne, K. Mercóla, M. Bar-Eli, M. J. Cline. Gene therapy techniques: Use of drug resistance selections in intact animals to insert human globin genes into bone marrow cells in living mice. In Molecular Mechanisms of Hemoglobin Switching ( G. Stamatoyannopoulos and A.W. Nienhuis, Eds.) New York, Grune & Stratton (1981).Google Scholar
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    Bar-Eli, M., K. E. Mercóla, D. J. Slamon, N. Mauritzson, H. D. Stang, M. J. Cline. Insertion of drug resistance genes in animals. Cell. Physiol, (suppl.) 1: 213–217 (1982).CrossRefGoogle Scholar
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    Shimotohno, K., and H. Temin. Formation of infectious progeny virus after insertion of herpes simplex thymidine kinase gene into DNA of an avian retrovirus. Cell 26: 67–77 (1981).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • Martin J. Cline
    • 1
  1. 1.Center for the Health SciencesUniversity of California School of MedicineLos AngelesUSA

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