Research on Gene Therapy
I would like to begin by describing the methods that are currently available for introducing new genes into mammalian cells. These techniques have generally been applied to cells in tissue culture. Historically, the oldest method for insertion of genes into cells utilizes chromosomes as the mechanism of gene transfer. A variety of chromosomal techniques have been described , Recipient and donor cells can be fused together; chromosomes can be isolated and incubated with target cells for endocytosis; and finally, chromosomes can be entrapped within a nuclear membrane and the membrane can then be fused to the target cell. None of the chromosomal techniques are very efficient at gene transfer; and, in general, only about one cell in ten million is transformed. By transformed, I mean that the target cell acquires new genetic information and expresses that information for some period of time.
KeywordsBone Marrow Cell Thymidine Kinase Globin Gene Dihydrofolate Reductase Helper Virus
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