InVitro DNA Synthesis as a Tool to Analyze and Alter Genes

  • Joachim Messing
Part of the Basic Life Sciences book series

Abstract

This overview describes some of the biochemical procedures which are used to analyze the primary structure of genetic information. This is one of the first steps in engineering genetic material so that it can be used for the production of materials in the medical, agricultural, and chemical industries. Another important step is the design and construction of synthetic genetic alterations either to explore function or to alter function in the sense of making a better product, e.g., yield, stability, higher activity, or specificity.

Keywords

Electrophoresis 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Gardner, R. C., Howarth, A. J, Hahan, P., Brown-Luedi, M., Shepherd, R. J., Messing J. The complete nucleotide sequence of an infectious clone of cauliflower mosaic virus by M13mp7 shotgun sequencing. Nucl. Acids Res. 9: 2871–2888 (1981).CrossRefGoogle Scholar
  2. 2.
    Goulian, M., Romberg,A., Sinsheimer, R. L. Enzymatic synthesis of DNA. XXIV. Synthesis of infectious phage ϕX174 DNA. Proc. Natl. Acad. Sci., U.S.A., 58: 2321–2328 (1967).Google Scholar
  3. 3.
    Heidecker, G., Messing, J., Gronenborn, B. A versatile primer for DNA sequencing in the M13mp2 cloning system. Gene 10: 69–73 (1980).CrossRefGoogle Scholar
  4. 4.
    Hutchinson, C.A., III, S. Phillips, M.H. Edgell, S. Gillam, P. Jahnke, and M. Smith. Mutagenesis at a specific position in a DNA sequence. J. Biol. Chem., 253: 6551–6560 (1978).Google Scholar
  5. 5.
    Larson, R. and J. Messing. Apple II software for M13 shotgun DNA sequencing. Nucl. Acids Res. 10: 39–49 (1982).CrossRefGoogle Scholar
  6. 6.
    Messing, J. An integrative strategy of DNA sequencing and experiments beyond. In Genetic Engineering: Principles and Methods (J. Setlow and A. Hollaender, eds), Vol 4, Plenum Press, New York, pp 19–36 (1982).Google Scholar
  7. 7.
    Messing, J. New M13 vectors for cloning, In Methods in Enzymology (R. Wu, ed). Academic Press, New York (in press, 1982).Google Scholar
  8. 8.
    Messing, J. Crea, R. Seeburg, P. H. A system for shotgun DNA sequencing. Nucl. Acids Res., 9: 309–321 (1981).CrossRefGoogle Scholar
  9. 9.
    Messing, J., Gronenborn, B., Müller-Hill, B., Hof, Schneider, P. H. Filamentous coliphage M13 as a cloning vehicle: Insertion of a Hind II fragment of the lac regulatory region into M13 replicative form in vitro. Proc. Natl. Acad. Sci., U.S.A., 74: 3642–3646 (1977).Google Scholar
  10. 10.
    Sanger, F., S. Nicklen, A. R. Coulson. DNA sequencing with chain terminating inhibitors. Proc. Natl. Acad. Sci., U.S.A., 74: 5463–5467 (1977).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • Joachim Messing
    • 1
  1. 1.Department of BiochemistryUniversity of MinnesotaSt. PaulUSA

Personalised recommendations