Some Characteristics of Non-Immunoglobulin Components in Low pH Extracts of A Non-Lymphoid Tumor

  • Maya Ran
  • Margot Lakonishuk
  • Esther Bachar
  • Isaac P. Witz
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 57)


In situ humoral immunity as an indispensable component of host-tumor relationship is being studied in our laboratory using various tumor systems (1–3). It is now well established that immunoglobulins localize in vivo within the tumor tissue of man and animals (3). Some of these tumor-associated Ig molecules may be anti tumor antibodies induced in tumor-bearers (e. g.2,4).


Tumor Extract Mouse IgG2a Elution Procedure Antitumor Antibody Anti Tumor Antibody 
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  1. 1.
    M. Ran and I.P. Witz, Tumor-associated immunoglobulins. The elution of IgG2 from mouse tumors, Int. J. Cancer 6: 361 (1970).CrossRefGoogle Scholar
  2. 2.
    M. Ran, G. Klein and I.P. Witz, Tumor-bound immunoglobulins. Evidence for the in vivo coating of tumor cells by potentially cytotoxic antitumor antibodies, Int. J. Cancer 4: 90 (1976).CrossRefGoogle Scholar
  3. 3.
    I.P. Witz, Tumor-bound immunoglobulins, in situ expressions of humoral immunity, Advances in Cancer Research 15:95 (1977).Google Scholar
  4. 4.
    T. Nethanel, R. Kinsky, N. Moav, R. Brown, M. Ran and I.P. Witz, Separation of tumor seeking small lymphocytes and tumor cells using percoll velocity gradients, J. Immunol. Methods 41: 43 (1981).CrossRefGoogle Scholar
  5. 5.
    R. Ehrlich and I.P. Witz, The elution of antibodies from viable tumor cells, J. Immunol. Meth. 26: 345 (1979).CrossRefGoogle Scholar
  6. 6.
    I.P. Witz, N. Lee and G. Klein, Serologically detectable specific and cross-reactive antigens on the membrane of a polyoma virus-induced murine tumor, Int. J. Cancer 18: 243 (1976).CrossRefGoogle Scholar
  7. 7.
    G. Klein, B. Ehlin and I.P. Witz, Serological detection of a polyoma-tumor-associated membrane antigen, Int. J. Cancer 23: 683 (1979).CrossRefGoogle Scholar
  8. 8.
    N. Moav and I.P. Witz, Characterisation of immunoglobulin eluted from murine tumor cells: Binding patterns of cytotoxic anti-tumor IgG, J. Immunol. Meth. 22: 51 (1978).CrossRefGoogle Scholar
  9. 9.
    M. Ran, M. Yaakubowicz and I.P. Witz, Lymphocy to toxic autoantibodies eluted from in vivo propagating sarcoma cells of mice, J. Natl. Cancer Inst. 60: 1509 (1978).Google Scholar
  10. 10.
    M. Ran, M. Yaakubowicz, O. Amitai and I.P. Witz, Tumor-localising lymphocytotoxic antibodies, in: “Contemporary topics in immunobiology”, I.P. Witz and M.G. Hanna Jr., eds., Plenum Publishing Corp., p. 191 (1980).Google Scholar
  11. 11.
    R.S. Kerbel. Increased sensitivity of rosetting assay for Fc receptors obtained by using non-hemagglutinating monoclonal antibodies, J. Immunol. Meth. 34: 1 (1980).CrossRefGoogle Scholar
  12. 12.
    G.R. Braslawsky, D. Serban and I.P. Witz, Receptors for immune complexes on cells within a polyoma-virus-induced murine sarcoma, Eur. J. Immunol. 6: 579 (1976).CrossRefGoogle Scholar
  13. 13.
    G.R. Braslawsky, M. Ran and I.P. Witz, Tumor-bound immunoglobulins: The relationship between the in vivo coating of tumor cells by potentially cytotoxic anti-tumor -antibodies and the expression of immune complex receptors, Int. J. Cancer 18: 116 (1976).CrossRefGoogle Scholar
  14. 14.
    A. Kulczycki Jr., R. Schneider, V. Krause, C. Chew Killian, L. Solanki and J.P. Atkinson, Purification of biologically- active Fcγ receptor from macrophages, Fed. Proc. 39: 799 (1980).Google Scholar
  15. 15.
    T. Suzuki, K. Hachimine and R. Sadasivan, Biochemical properties of biologically active FcY receptors isolated from human B lymphocytes, Fed. Proc. 39: 463 (1980).Google Scholar
  16. 16.
    K. James, S. Davis and J. Merriman, Immunological and biochemical characteristics of acid citrate eluates from tumor cells: a major non-immunoglobulin component, Brit. J. Cancer 43: 294 (1981).CrossRefGoogle Scholar
  17. 17.
    R.S. Kerbel and A.J.S. Davies, The possible biological significance of Fc receptors on mammalian lymphocytes and tumor cells. Cell 3: 105 (1974).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • Maya Ran
    • 1
  • Margot Lakonishuk
    • 1
  • Esther Bachar
    • 1
  • Isaac P. Witz
    • 2
  1. 1.Department of Microbiology, The George S. Wise Faculty of Life SciencesTel Aviv UniversityTel AvivIsrael
  2. 2.Incumbent: The David Furman Chair in Immunobiology of CancerUSA

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