Immune Recognition of Tumor Cells Detected by Lymphocyte Mediated Cytotoxicity

  • Eva Klein
  • Farkas Vanky
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 57)


The goal of human tumor immunology studies is to demonstrate the recognition of autologous tumor cells. Though the recognition may not ensure that the immune mechanisms can control tumor growth, increasing knowledge of the details in the immune events may lead to therapeutical exploitations. There is considerable interest in the establishment of tumor cell reactive T lymphocyte cultures. They would provide a tool for characterization of tumor-related antigens and may also be useful for therapeutical strategies.


Natural Killer Autologous Tumor Immune Recognition Autologous Tumor Cell Lytic Effect 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    B.M. Vose, F. Vánky, and E. Klein, Lymphocyte cytotoxicity against autologous tumor biopsy cells in humans. Int. J. Cancer 20: 512 (1977).CrossRefGoogle Scholar
  2. 2.
    F. Vánky, J. Stjernswärd, and I. J. Nilsonne, Cellular immunity to human sarcoma. J. Natl. Cancer Inst. 46: 1145 (1971).Google Scholar
  3. 3.
    F. Vánky, B.M. Vose, M. Fopp, and E. Klein, Human tumor-lymphocyte interaction in vitro. VI. Specificity of primary and secondary autologous lymphocyte-mediated cytotoxicity. J. Natl. Cancer Inst. 62: 1407 (1979).Google Scholar
  4. 4.
    F. Vánky, S. Argov, S. Einhorn, and E. Klein, Role of alloantigen’s in natural killing. Allogeneic but not autologous tumor biopsy cells are sensitive for interferon-induced cytotoxicity of human blood lymphocytes. J. Exp. Med. 151: 1151 (1980).CrossRefGoogle Scholar
  5. 5.
    F. Vánky, S. Argov, and E. Klein, Tumor biopsy cells participating in systems in which cytotoxicity of lymphocytes is generated. Autologous and allogeneic studies. Int. J. Cancer 27: 273 (1981).CrossRefGoogle Scholar
  6. 6.
    B.M. Vose, P. Vánky, M. Fopp, and E. Klein, In vitro generation of a secondary cytotoxic response against autologous human tumor biopsy cells. Int. J, Cancer 21: 288 (1978).CrossRefGoogle Scholar
  7. 7.
    E. Vánky, T. Gorsky, Y. Gorsky, M.G. Masucci, and E. Klein, Lysis of tumor biopsy cells by autologous T-lymphocytes activated in mixed cultures and propagated with TCGF. J. Exp. Med., in press.Google Scholar
  8. 8.
    J.L. Strausser, A. Mazumder, E.A. Grimm, M.T. Lotze, and S.A. Rosenberg, Lysis of human solid tumors by autologous cells sensitized in vitro to alloantigens. J. Immunol. 127: 266 (1931).Google Scholar
  9. 9.
    J.M. Zarling, P.C. Raich, M. McKeough, and F.H. Bach, Generation of cytotoxic lymphocytes in vitro against autologous human leukemia cells. Nature 262: 691 (1976).CrossRefGoogle Scholar
  10. 10.
    O.M. Landazuri, and R.B. Herberman, Specificity of cellular immune reactivity to virus induced tumors. Nature 238: 18 (1972).CrossRefGoogle Scholar
  11. 11.
    J. Kornbluth, and B. Dupond, Cloning and functional characterization of primary alloreactive human T lymphocytes. J. Exp. Med. 152: 164 (1980).Google Scholar
  12. 12.
    C. Taswell, H.R. MacDonald, and J.-C. Cerottini, Clonal analysis of cytotoxic T lymphocyte specificity. I. Phenotypically distinct sets of clones as the cellular basis of cross-reactivity to alloantigens. J. Exp. Med. 151: 1372 (1980).CrossRefGoogle Scholar
  13. 13.
    E. Vánky, and E. Klein, Alloreactive cytotoxicity of interferon triggered human lymphocytes detected with tumor biopsy targets. Immunogenetics, in press.Google Scholar
  14. 14.
    R.M. Zinkernagel, and P.C. Doherty, MHC-restricted cytotoxic T cells: studies on the biological role of polymorphic major transplantation antigens determining T cell restriction — specificity, function, and responsiveness. Adv. Immunol. 27: 51 (1979).CrossRefGoogle Scholar
  15. 15.
    D. Gillis, and K.A. Smith, Long-term culture of tumor-specific cytotoxic T cells. Nature (Lond.) 268: 154 (1977).CrossRefGoogle Scholar
  16. 16.
    J.L. Strausser, and S.A. Rosenberg, In vitro growth of cytotoxic human lymphocytes. I. Growth of cells sensitized in vitro to alloantigens. J. Immunol. 121: 1491 (1978).Google Scholar
  17. 17.
    T. Bakács, P. Gergely, and E. Klein, Characterization of cytotoxic human lymphocyte subpopulations. The role of Fc-receptor carrying cells. Cell. Immunol. 32: 317 (1977).CrossRefGoogle Scholar
  18. 18.
    T. Bakács, E. Klein, E., Yefenof, P. Gergely, and M. Steinitz, Human blood lymphocyte fractionation with special attention to their cytotoxic potential. Immunobiol. 154: 121 (1978).Google Scholar
  19. 19.
    M.A. Kail, and H.S. Koren, Heterogeneity of human natural killer populations. Cell. Immunol. 40: 58 (1978).CrossRefGoogle Scholar
  20. 20.
    H.D. Kay, G.D. Bonnard, W. H. West, and R.B. Herberman, A functional comparison of human Fc-receptor bearing lymphocytes active in natural cytotoxicity and antibody- dependent cellular cytotoxicity. J. Immunol. 118: 2058 (1977)Google Scholar
  21. 21.
    G. Masucci, M.G. Masucci, and E. Klein, Activation of human blood lymphocyte subsets for cytotoxic potential. To be published.Google Scholar
  22. 22.
    M.G. Masucci, G. Masucci, E. Klein, and W. Berthold, Target selectivity of interferon induced human killer lymphocytes related to their Pc receptor expression. Proc. Natl. Acad. Sci. 77: 3620 (1980).CrossRefGoogle Scholar
  23. 23.
    L. Ahrlund-Richter, E. Klein, and G. Masucci, Somatic hybrids between a high NK-sensitive lymphoid (YAC-IR) and several low sensitive sarcoma or L-cell derived mouse lines exhibit low sensitivity. Som. Cell Gen. 6: 89 (1980).CrossRefGoogle Scholar
  24. 24.
    E. Saksela, T. Timonen, and K. Cantell, Human natural killer activity is augmented by interferon via recruitment of “pre-NK” cells. Scand. J. Immunol. 10: 257 (1979).CrossRefGoogle Scholar
  25. 25.
    D. Santoli, and H. Koprowski, Mechanism of activation of human natural killer cells against tumor and virus infected cells. Immunol. Rev. 44: 125 (1979).CrossRefGoogle Scholar
  26. 26.
    S. Targan, and P. Dorey, Interferon activation of pre-spontaneous killer (pre-SK) cells and alteration in kinetics of lysis of both “pre-SK” and active SK cells. J. Immunol. 124: 2157 (1980).Google Scholar
  27. 27.
    M.G. Masucci, E. Klein, and S. Argov, Non-specific cytotoxic potential of human lymphocyte cultures is different from NK and its strength correlates to the level of blastogenesis. J. Immunol. 124: 2458 (1980).Google Scholar
  28. 28.
    A. Poros, and E. Klein, Cultivation with K562 cells leads to blastogenesis and increased cytotoxicity with changed properties of the active cells when compared to fresh lymphocytes. Cell. Immunol. 41: 240 (1978).CrossRefGoogle Scholar
  29. 29.
    J.K. Seeley, G. Masucci, A. Poros, E. Klein, and S.H. Golub, Studies on cytotoxicity generated in human mixed lymphocyte cultures. J. Immunol. 123: 1303 (1979).Google Scholar
  30. 30.
    R.I.H. Bolhuis, and C.P.M. Ronteltalp, Generation of natural killer (NK) cell activity after mixed lympho-cyte culture (MLC). Activation of effector cells in NK depleted populations. Immunology Letters 1:191 (1960).CrossRefGoogle Scholar
  31. 31.
    D.M. Callewaert, J.J. Lightbody, J. Kaplan, J. Joroszewski, W.D. Peterson, and J.C. Rosenberg, Cytotoxicity of human peripheral lymphocytes in cell-mediated lympholysis; antibody dependent cell-mediated lympholysis and natural cytotoxicity assays after mixed lymphocyte culture. J. Immunol. 121: 81 (1978).Google Scholar
  32. 32.
    J.V. Zielska, and S.H. Golub, Petal calf serum induced blastogenic and cytotoxic response of human lymphocytes. Cancer Res. 36: 3842 (1976).Google Scholar
  33. 33.
    M.R. Martin Chandon, P. Vanky, C. Carnaud, and E. Klein, In vitro education on autologous human sarcoma generates non-specific killer cells. Int. J. Cancer 15: 342 (1975).CrossRefGoogle Scholar
  34. 34.
    K. Tomonari, Cytotoxic T cells generated in the autologous mixed lymphocyte reaction. I. Primary autologous mixed lymphocyte reaction. J. Immunol. 124: 1111 (1980).Google Scholar
  35. 35.
    E.A. Svedmyr, P. Deinhart, and G. Klein, Sensitivity of different target cells to the killing action of peripheral lymphocytes stimulated by autologous lymphoblastoid cell lines. Int. J. Cancer 13: 891 (1974).CrossRefGoogle Scholar
  36. 36.
    J.M. Zarling, and P.C. Kung, Monoclonal antibodies which distinguish between human NK cells and cytotoxic T lymphocytes. Nature 288: 394 (1980).CrossRefGoogle Scholar
  37. 37.
    G. Masucci, A. Poros, J.K. Seeley, and E. Klein, In vitro generation of K562 killers in human T-lymphocyte sub-sets. Cell. Immunol. 52: 247 (1980).CrossRefGoogle Scholar
  38. 38.
    L.M. Callewaert, J. Kaplan, D.P. Johnson, and W. D. Jr. Peterson, Spontaneous cytotoxicity of cultured human cell lines mediated by normal peripheral lymphocytes. II. Specificity for target antigens. Cell. Immunol. 42: 103 (1979).CrossRefGoogle Scholar
  39. 39.
    E. Klein, Natural and activated cytotoxic T lymphocytes. Immunology Today 1: IV (1980).Google Scholar
  40. 40.
    E. Klein, and P. Vánky, Natural and activated lymphocytes which act on autologous and allogeneic tumor cells. Cancer Immunol. Immunother. 11: 183 (1981).CrossRefGoogle Scholar
  41. 41.
    H. Wagner, and M. Röllinghof, T-T cell interactions during in vitro cytotoxic allograft responses. I. Soluble products from activated Lyl + T cells triggered autonomously by antigen primed Ly2,3 + T cells to cell proliferation and cytotoxic activity. J. Exp. Med. 148: 1523 (1978).CrossRefGoogle Scholar
  42. 42.
    H. Kobayashi, M. Hoshokova, and T. Oikawa, Transplantation immunity to syngeneic tumors in WKR rats immunized with allogeneic cells. Transpl. Proc. 12: 156 (1980).Google Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • Eva Klein
    • 1
  • Farkas Vanky
    • 1
  1. 1.Department of Tumor BiologyKarolinska InstitutetStockholmSweden

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