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Reverse Transcriptase: A Molecular Probe for the Expression of Retroviral Information in Human Tumors

  • Prakash Chandra
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 57)

Abstract

The discovery of reverse transcriptase in RNA tumor viruses (Temin and Mizutani, 1970; Baltimore, 1970), implicated by the provirus hypothesis of Temin (Temin, 1964a, 1964b), was a new dimension in the understanding of how such viruses multiply and moreover, how do they catalyze neoplastic transformation. Soon after the discovery of reverse transcriptase in retroviruses, Gallo and his associates detected a similar enzymic activity in lymphoblasts of patients with leukemia (Gallo et al., 1970). This activity was absent in mitotically stimulated normal human peripheral blood leucocytes. The first detection of intracellular reverse transcriptase in leukemic cells (Gallo et al., 1970) provided incentive to examine this activity in other human tumor tissues (Chandra, 1979; Chandra and Steel, 1977, 1980; Chandra et al., 1975, 1978, 1980, 1981; Ebener et al., 1979? Ohno et al., 1977; Poiesz et al., 1980; Welte et al., 1979; and Witkin et al., 1975). The main objective of these studies was: 1) to understand whether this is a new enzyme or is simply a modified form of one of the constitutive cellular DNA polymerases α, β and γ, 2) to develop new procedures of purifying this viral-like reverse transcriptase from neoplastic tissues, and 3) to characterize the purified reverse transcriptase from human tumor tissues to determine whether the detection of this enzyme indicates an expression of retrovirus information in these tissues.

Keywords

Reverse Transcriptase Reverse Transcriptase Activity Granulocytic Sarcoma Orbital Tumor Reverse Trans 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Balda, B.R., Hehlmann,R., Cho,J.R. and Spiegelman,S. (1975): Proc. Natl. Acad. Sci. U.S.A. 72, 3697–3700.CrossRefGoogle Scholar
  2. Baltimore, D. (1970): Nature, Lond. 226, 1209–1211.CrossRefGoogle Scholar
  3. Cavdar, A.O., Arcasoy,A., Gözdasoglu,S. and Demirag,B. (1971): Lancet I, 680–682.Google Scholar
  4. Cavdar, A.O., Arcasoy,A., Babcan,E., Gözdasoglu,S., U. Topuz and Fraumeni,J.F., Jr. (1978): Cancer 41, 1606–1609.CrossRefGoogle Scholar
  5. Chandra,P. (1979): In, Proceedings of the XIIth Intern. Cancer Congress, Buenos Aires (Edt. G.P. Margison), Vol. 1, 29–41, Pergamon Press, Oxford and New York.Google Scholar
  6. Chandra,P. (1979a): In, Proceedings of the Federal of European Biochemical Societies Meeting, Dubrovnik, (Edt. P. Mildner) Vol 61, 215–227, Pergamon Press.Google Scholar
  7. Chandra,P. and Steel,L.K. (1977): Biochem. J. (Lond.) 167, 513–524.Google Scholar
  8. Chandra,P. and Steel, L.K. (1980): Cancer Lett. 9, 67–74.CrossRefGoogle Scholar
  9. Chandra,P., Steel,L.K., Laube,H. and Kornhuber, B. (1975): FEBS-Lett. 58, 71–75.CrossRefGoogle Scholar
  10. Chandra,P., Balikcioglu,S., and Mildner,B. (1978): Cancer Lett. 5, 299–310.CrossRefGoogle Scholar
  11. Chandra,P., Steel,L.K., Laube,H. and Kornhuber,B. (1979): In, Antiviral Mechanisms in the Control of Neoplasia, (Edt. P. Chandra) pp 177–198, Plenum Press, N.Y.Google Scholar
  12. Chandra,P., Steel,L.K., Laube,H., Balikcioglu,S., Ebener, U. and Welre,K. (1979a): In, Proceedings of the 8th Internatl. Symposium on the Biological Characterization of Human Tumors, Athens, Excerpta Medica, Amsterdam, pp.Google Scholar
  13. Chandra,P., Steel,L.K. and Cavdar, A.O. (1979b): In, Modern Trends in Human Leukemia, Vol. III, (Edts. R. Neth Et al.), pp 497–500, Springer-Verlag, Berlin-N.Y.Google Scholar
  14. Chandra,P., Steel,L.K., Laube,H., Balikcioglu,S., Mildner B., Ebener,U., Welte,K. and Vogel,A. (1980): Cold Spring Harbor Conf. Cell Proliferation 7, 775–791.Google Scholar
  15. Chandra,P., Balikcioglu,S. and Mildner,B. (1981): Cell, and Molec. Biol. ( Pergamon Press ) 27, 239–251.Google Scholar
  16. Ebener,U., Welte,K. and Chandra,P. (1979): Cancer Lett. 7, 179–188.CrossRefGoogle Scholar
  17. Gallo,R.C., Yang,S.S. and Ting,R.C. (1970): Nature(Lond.) 228, 927–929.CrossRefGoogle Scholar
  18. Gallo,R.C., Gallaghar,R.E., Miller,N.R., Mondal,H., Saxinger,W.C., Mayer,R.T., Smith,R.G. and Gillespie, D.H. (1975): Cold Spring Harbor Symp. Quant. Biol. 39, 933–961.CrossRefGoogle Scholar
  19. Gillespie,D., Saxinger,W.C. and Gallo,R.C. (1975): Progr. Nucl.Acid Res. Mol. Biol. 15, 1–108.CrossRefGoogle Scholar
  20. McAllister, R.M., Nelson, Ress, W.A., Johnson, E.Y., Rongey, R.W. and Gardner, M.B. (1971): J. Natl. Cancer Inst. 47, 603–611.Google Scholar
  21. McAllister, R.M., Nicolson, M., Gardner, M.B., Rongey, R.W., Rasheed, S., Sarma, P.S., Huebner, R.J., Hatanaka, M., Oroszlan,S., Gilden, R.V., Kabigiting, A. and Vernon, L. (1972): Nature New Biol. 235, 3–6.Google Scholar
  22. Nelson, J., Leong, J.A. and Levy, J.A. (1978): Proc.Natl. Acad.Sci. U.S.A. 75, 6263–6267.CrossRefGoogle Scholar
  23. Ohno, T. and Spiegelman, S. (1977): Proc.Natl.Acad.Sci. U.S.A. 74, 2144–2148.CrossRefGoogle Scholar
  24. Ohno, T., Sweet, R.W., Hu, R., Dejak, D. and Spiegelman, S. (1977): Proc.Natl.Acad.Sci. U.S.A. 74, 764–768.CrossRefGoogle Scholar
  25. Poiesz, B.J., Ruscetti, E.W., Gazdar, A.F., Bunn, P.A., Minna, J.D. and Gallo, R.C. (1980): Proc.Natl.Acad.Sci. U.S.A. 77, 7415–7419.CrossRefGoogle Scholar
  26. Sarin, P., Friedman, B. and Gallo, R.C. (1977): Biochem. Biophys.Acta 479, 198–206.CrossRefGoogle Scholar
  27. Temin, H. (1964a): Natl.Cancer Inst. Monograph 17,55 7–570.Google Scholar
  28. Temin, H. (1964b): J. Virol. 23, 486–494.CrossRefGoogle Scholar
  29. Temin, H. (1971a): J. Nazi.Cancer Inst. 42, 11Google Scholar
  30. Temin, H. (1971b): J. Natl.Cancer Inst. 46, III-VIIIGoogle Scholar
  31. Temin, H. (1972): Proc.Natl.Acad.Sci. U.S.A. 69, 1016–1020CrossRefGoogle Scholar
  32. Temin, H. (1974a): Cancer Res. 34, 2835–2841.Google Scholar
  33. Temin, H. (1974b): Annual Rev. Genetics 8, 155–176.CrossRefGoogle Scholar
  34. Temin, H. and Mizutani,S.(1970): Nature(Lond.) 226,1211–1214.CrossRefGoogle Scholar
  35. Vogel, A. and Chandra, P. (1981): Biochem.J. (Lond) 197, 553–563.Google Scholar
  36. Welte, K., Ebener, U. and Chandra, P. (1979): Cancer Lett. 7, 189–195.CrossRefGoogle Scholar
  37. Witkin, S.S., Ohno, T. and Spiegelman, S. (1975): Proc. Natl. Acad.Sci. U.S.A. 72, 4133–4136.CrossRefGoogle Scholar
  38. Wong-Staal, F., Gillespie, D.H. and Gallo, R.C. (1976): Nature(Lond.) 262, 190–195.CrossRefGoogle Scholar
  39. Wu, A. and Gallo, R.C. (1975): CRC Crit.Rev.Biochem. 1975 289–347.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • Prakash Chandra
    • 1
    • 2
  1. 1.Center of Biological ChemistryUniversity Medical SchoolFrankfurtWest Germany
  2. 2.Laboratory of Molecular BiologyUniversity Medical SchoolFrankfurtWest Germany

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