Properties of an RNA Retrovirus Continuously Produced at Low Levels by a Human Histiocytic Lymphoma Cell Line

  • Henry S. Kaplan
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 57)


Type-C RNA viruses (retroviruses) are capable of inducing leukemias, lymphomas, and sarcomas in several mammalian species, including mice, cats, guinea pigs, cattle, and subhuman primates (Kaplan 1974), suggesting that they may also be involved in the etiology of these types of neoplasms in man. Retroviruses have been detected in culture fluids after short-term culture of hematopoietic cells of patients with leukemias (Gallagher and Gallo, 1975; Nooter et al.,1975; Teich et al., 1975) and of diploid human embryonic cell strains (Panem et al., 1975).


Lymphoma Cell Line Reverse Transcriptase Activity Peripheral Blood Mononuclear Histiocytic Lymphoma Supernatant Culture Fluid 
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  1. Baxt, W., Hehlmann, R., and Spiegelman, S., 1972, Human leukaemic cells contain reverse transcriptase associated with a high molecular weight virus-related RNA, Nat. New Biol. 240: 72.CrossRefGoogle Scholar
  2. Baxt, W., Yates, J.W., Wallace, H.J. Jr., Holland, J.F., and Spiegelman, S., 1973, Leukemia-specific DNA sequences in leukocytes of the leukemic member of identical twins, Proc. Nat. Acad. Sci. USA, 70: 2629.CrossRefGoogle Scholar
  3. Chan, E., Peters, W.P., Sweet, R.W., Ohno, T., Kufe, D.W., Spiegelman, S., Gallo, R.C., and Gallagher, R.E., 1976, Characterization of a virus (HL23V) isolated from cultured acute myelogenous leukaemic cells, Nature, 2: 266.CrossRefGoogle Scholar
  4. Chezzi, C., Dettori, G., Manzari, V., Agliano, A.M., and Sanna, A., 1976, Simultaneous detection of reverse transcriptase and high molecular weight RNA in tissue of patients with Hodg¬kin1 s disease and patients with leukemia. Proc. Natl. Acad. Sci. USA, 73: 4649.CrossRefGoogle Scholar
  5. Dalton, A.J., 1972, RNA tumor viruses: Terminology and ultrastructural aspects of virion morphology and replication, J. Natl. Cancer Inst. 49: 323.Google Scholar
  6. Epstein, A.L., and Kaplan, H.S., 1974, Biology of the human malignant lymphomas. I. Establishment in continuous cell culture and hetero-transplantation of diffuse histiocytic lymphomas, Cancer 34: 1851.CrossRefGoogle Scholar
  7. Epstein, A.L., Henle, W., Henle, G., Hewetson, J.F., and Kaplan, H. S., 1976a, Surface marker characteristics and Epstein-Barr virus studies of two established North American Burkitt’s lymphoma cell lines, Proc. Nat. Acad. Sci. USA, 73: 228.CrossRefGoogle Scholar
  8. Epstein, A.L., Herman, M.M., Kim, H., Dorfman, R.F., and Kaplan, H.S., 1976b, Biology of the human malignant lymphomas. III. Intracranial transplantation in the nude, athymic mouse, Cancer, 37: 2158.CrossRefGoogle Scholar
  9. Gallagher, R.E., and Gallo, R.C., 1975, Type C RNA virus tumor isolated from cultured human acute myelogenous leukemia cells, Science, 187: 350.CrossRefGoogle Scholar
  10. Gallo, R.C., Miller, N.R., Saxinger, W.C., and Gillespie, D., 1972, Primate RNA tumor virus-like DNA synthesized endogenously by RNA — dependent DNA polymerase in virus-like particles from fresh human acute leukemic blood cells, Proc. Nat. Acad. Sci. USA, 70: 3219.CrossRefGoogle Scholar
  11. Goodenow, R,S,, Brown, S., Levy, R., and Kaplan, H.S., 1980, Partial characterization of the virus proteins of a type-C RNA virus produced by a human histiocytic lymphoma cell line, in: “Viruses in Naturally Occurring Cancers,” M. Essex, G. Todaro, and H. zur Hausen, eds., Cold Spring Harbor Laboratory, New York, pp. 737–752.Google Scholar
  12. Goodenow, R.S., and Kaplan, H.S., 1979, Characterization of the reverse transcriptase of a type C RNA virus produced by a human lymphoma cell line, Proc. Nat. Acad. Sci. USA, 76: 4971–4975.CrossRefGoogle Scholar
  13. Goodenow, R.S., Liu, S, Fry, K., Levy, R., and Kaplan, H.S., 1981, Expression of C-type RNA viral proteins by a human lymphoma cell line, in: “Recent Advances in Malignant Lymphomas: Etiology, Immunology, Pathology, Treatment,” S.A. Rosenberg and H.S. Kaplan, Eds., Academic Press, New York, 1981, in press.Google Scholar
  14. Hall, W.T., and Schidlovsky, 1976, Typical Type-C virus in human leukemia, J. Natl. Cancer Inst., 56: 639.Google Scholar
  15. Kaiser-McCaw, B., Epstein, A.L., Kaplan, H.S., and Hecht, F., 1977, Chromosome 14 translocation in African and North American Burkitt’s lymphoma, Int. J. Cancer, 19: 482.CrossRefGoogle Scholar
  16. Kalyanaraman, V.S., Sarngadharan, M.G. Poiesz, B., Ruscetti, F., and Gallo, R., 1981, Immunological properties of a type C retrovirus isolated from cultured human T-lymphoma cells and comparison to other mammalian retroviruses, J. Virol., 38: 906.Google Scholar
  17. Kaplan, H.S., 1974, Leukemia and lymphoma in experimental and domestic animals, Ser. Haematol., 7: 94.Google Scholar
  18. Kaplan, H.S., 1978, Studies of an RNA virus isolated from a human histiocytic lymphoma cell line, in: “Differentiation of Normal and Neoplastic Hematopoietic Cells,” B. Clarkson, P.A. Marks, and J.E. Till, eds., Cold Spring Harbor Laboratory, New York.Google Scholar
  19. Kaplan, H.S., Goodenow, R.S., Epston, A.L., Gartner, S., Decleve, A., and Rosenthal, P.N., 1977, Isolation of a C RNA virus from an established human histiocytic lymphoma cell line, Proc. Nat. Acad. Sci. USA, 74: 2564.CrossRefGoogle Scholar
  20. Kaplan, H.S., Goodenow, R.S., Gartner, S., and Bieber, M., 1979, Biology and virology of the human malignant lymphomas: 1st Milford D. Schulz lecture, Cancer 43: 1CrossRefGoogle Scholar
  21. Klement, V., Rowe, P., Hartley, J.W., and Pugh, W.E., 1969, Mixed culture cytopathogenicity: A new test for growth of murine leukemia viruses in tissue culture, Proc. Nat. Acad. Sci. USA, 63: 753.CrossRefGoogle Scholar
  22. Klucis, E., Jackson, L., and Parsons, P.G., 1976, Survey of human lymphoblastoid cell lines and primary cultures of normal and leukemic leukocytes for oncornavirus production, Int. J. Cancer, 18: 413.CrossRefGoogle Scholar
  23. Nooter, K., Aarssen, A.M., Bentvelzen, P., De Groot, F.G., and van Peit, F.G., 1975, Isolation of infectious C-type oncorna-virus from human leukaemic bone marrow cells, Nature, 256: 595.CrossRefGoogle Scholar
  24. Okabe, H., Gilden, R.V., Hatanaka, M., Stephenson, J.R., Gallagher, R.E., Gallo, R.C., Tronick, S.R., and Aaronson, S.A., 1976, Immunological and biochemical characterization of the type C viruses isolated from cultured human AML cells, Nature, 260: 264.CrossRefGoogle Scholar
  25. Panem, S., Prochownik, E.V., Reale, F.R., and Kirsten, W.H., 1975, Isolation of type C virions from a normal human fibroblast strain, Science, 189: 297.CrossRefGoogle Scholar
  26. Poiesz, B.J., Ruscetti, F.W., Gazdar, A.F., Bunn, P.A., Minna, J.D., and Gallo, R.C., 1980, Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma, Proc. Nat. Acad. Sci. USA, 77: 7415.CrossRefGoogle Scholar
  27. Poiesz, B.J, Ruscetti, F.W., Mier, J.W., Woods, A.M., and Gallo, R. C., 1980, T-cell lines established from human T-lympocytic neoplasias by direct response to T-cell growth factor, Proc. Nat. Acad. Sci. USA, 77: 6815.CrossRefGoogle Scholar
  28. Rangan, S.R.S., 1974, C-type oncogenic viruses of nonhuman primates, Lab. Anim. Sci., 24: 193.Google Scholar
  29. Rangan, S.R.S., Moyer, P.P., Cheong, M.P., and Jensen, E.M., 1972a, Detection and assay of feline leukemia virus ( FeLV) by a mixed-culture cytopathogenicity method, Virology, 47: 247.CrossRefGoogle Scholar
  30. Rangan, S.R.S., Wong, M.C., Ueberhorst, P.J., and Ablashi, D.V., 1972b, Mixed culture cytopathogenicity induced by virus preparations derived from cultures infected by simian sarcoma virus, J. Natl. Cancer Inst., 49: 571.Google Scholar
  31. Reitz, M.S., Poiesz, B., Ruscetti, F.W., and Gallo, R.,1981, Characterization and distribution of nucleic acid sequences of a novel retrovirus isolated from neoplastic human T-lymphocytes, Proc. Nat. Acad. Sci. USA, 78: 1887.CrossRefGoogle Scholar
  32. Rho, H.M., Poiesz, B., Ruscetti, F.W., and Gallo, R.C., 1981, Characterization of the reverse transcriptase from a new retrovirus ( HTLV) produced by a human cutaneous T-cell lymphoma cell line, Virology, 112: 355.CrossRefGoogle Scholar
  33. Scott, M.L., McKereghan, K., Kaplan, H.S., and Fry, K.E., Molecular cloning and partial characterization of unintegrated gibbon ape leukemia virus DNA, Proc. Nat. Acad. Sci. USA, 78: 4213.Google Scholar
  34. Teich, N.M., Weiss, R.A., Salahuddin, S.Z., Gallagher, R.E., Gillespie, D.H., and Gallo, R.C., Infective transmission and characterization of a C-type virus released by cultured human myeloid leukaemia cells, Nature, 256: 551.CrossRefGoogle Scholar
  35. Witkin, S.S., Ohno, T., and Spiegelman, S., 1975, Purification of RNA-instrueted DNA polymerase from human leukemic spleens, Proc. Nat. Acad. Sci. USA, 72: 4133.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • Henry S. Kaplan
    • 1
    • 2
  1. 1.Cancer Biology Research LaboratoryStanford University School of MedicineStanfordUSA
  2. 2.Department of RadiologyStanford University School of MedicineStanfordUSA

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