Advertisement

Transformation-Enhancing Factors and Fibronectin Degradation Products as Possible Tumour Markers

  • Sergio Barlati
  • Giuseppina De Petro
  • Tapio Vartio
  • Antti Vaheri
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 57)

Abstract

The medium of chick embryo fibroblasts (CEF) transformed by Schmidt-Ruppin Strain of Rous sarcoma virus (SR-RSV) contains factors which complement the expression of some transformation parameters depending on the src gene.

Keywords

Rous Sarcoma Virus Chicken Embryo Fibroblast Newborn Calf Serum Chick Embryo Fibroblast Fibronectin Fragment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    S. Barlati, C. Kryceve and P. Vigier, Stimulation of transformation by factor(s) present in the medium of Rous Sarcoma Virus transformed cells, Abstracts XIth Int. Cancer Congress, 86 (1974).Google Scholar
  2. 2.
    C. Kryceve, P. Vigier and S. Barlati, Transformation enhancing factor(s) produced by virus-transformed and established cells, Int. J. Cancer. 17: 370 (1976).CrossRefGoogle Scholar
  3. 3.
    C. Kryceve, J. M. Biquard, S. Barlati, D. Lawrence and P. Vigier, Characteristics of the transformation enhancing factor(s) produced by RSV-transformed and other transformed cells, in: “Avian RNA Tumour Viruses”, S. Barlati and C. De Giuli, ed., Piccin Medical Books, Padua (1978).Google Scholar
  4. 4.
    C. Kryceve-Martinerie, J. M. Biquard, D. Lawrence, P. Vigier, S. Barlati and P. Mignatti, Transformation-enhancing factor(s) released from chicken Rous sarcoma cells: effect on some transformation parameters, Virology. 112: 436 (1981).CrossRefGoogle Scholar
  5. 5.
    S. Barlati, P. Mignatti, A. Brega, G. De Petro and E. Ascari, Utilization of Rous sarcoma virus for the detection of transformation enhancing and inhibiting factors in human plasma, in: “Avian RNA Tumour Viruses”, S. Barlati and C. De Giuli, ed., Piccin Medical Books, Padua (1978).Google Scholar
  6. 6.
    P. Mignatti, E. Ascari and S. Barlati, Potential diagnostic and prognostic significance of the transformation-enhancing factor(s) in the plasma cryoprecipitate of tumour patients, Int. J. Cancer. 25: 727 (1980).CrossRefGoogle Scholar
  7. 7.
    S. Barlati and P. Mignatti, Transformation enhancing factors in the plasma of cancer patients, in: “Control Mechanisms in Animal Ceils”, L. Jimenez de Asua et al. ed., Raven Press, New York (1980).Google Scholar
  8. 8.
    G. De Petro, S. Barlati, T. Vartio and A. Vaheri, Transformation-enhancing activity of gelatin-binding fragments of fibronectin, Proc. Natl. Acad. Sci. U.S.A. 78: 4965 (1981).CrossRefGoogle Scholar
  9. 9.
    S. Barlati, G. De Petro, T. Vartio and A. Vaheri, Role of fibronectin degradation products on cellular transformation, in.: “Protides of the Biolgical Fluids”, E. Peeters, ed., Pergamon Press (in press) (1981).Google Scholar
  10. 10.
    A. Vaheri, E. Ruoslahti and D. F. Mosher, (edst) “Fibroblasts Surface Protein”, Ann. Acad. Sci., New York (1978).Google Scholar
  11. 11.
    T. Vartio, H. Seppa and A. Vaheri, Susceptibility of soluble and matrix fibronectins to degradation by tissue proteinases, mast cell chymase and cathepsin G, J. Biol. Chem. 256: 471 (1981).Google Scholar
  12. 12.
    J. M. Biquard and P. Vigier, Characteristics of a conditional mutant of Rous sarcoma virus defective in the ability to transform cells at high temperature, Virology. 4: 444 (1972).CrossRefGoogle Scholar
  13. 13.
    S. Barlati, C. Kryceve and P. Vigier, Different stages of transformation of Rous virus-infected cells evidenced by the methylene blue staining, Intervirology. 4: 23 (1974).CrossRefGoogle Scholar
  14. 14.
    N. M. Blackett, Statistical accuracy to be expected from cell colony assays; with special reference to the spleen colony assay, Cell Tissue Kinet. 7: 407 (1974).Google Scholar
  15. 15.
    E. Engvall and E. Ruoslahti, Binding of soluble form of fibroblast surface protein, fibronectin to collagen, Int. J. Cancer. 20: 1 (1977).CrossRefGoogle Scholar
  16. 16.
    M. Vuento and A. Vaheri, Purification of fibronectin from human plasma by affinity chromatography under nondenaturing conditions, Biochem. J. 183: 331 (1979).Google Scholar
  17. 17.
    U. K. Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature. 227: 680 (1970).CrossRefGoogle Scholar
  18. 18.
    H. Towbin, T. Staehelin and J. Gordon, Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications, Proc. Natl. Acad. Sci. U.S.A. 76: 4350 (1979).CrossRefGoogle Scholar
  19. 19.
    S. Stenman and A. Vaheri, Fibronectin in human solid tumours, Int. J. Cancer. 27: 427 (1981).CrossRefGoogle Scholar
  20. 20.
    J. P. Quigley, Proteolytic enzymes of normal and malignant cells, in: “Surface of Normal and Malignant cells”, R. Hynes, ed., John Wiley & Sons New York (1979).Google Scholar
  21. 21.
    H. D. Todd, M. S. Coffee, T. P. Vaalkes, M. D. Abeloff and R. G. Parsons, Serum levels of fibronectin and a fibronectin-1ike DNA-binding protein in patients with various diseases, J. Natl. Cancer Inst. 65: 901 (1980).Google Scholar
  22. 22.
    K. Huber, J. Kirchheimer and B. R. Binder, Rapid isolation of native urokinase from normal human urine, Thrombos. Haemostas. 46: 11 (1981).Google Scholar
  23. 23.
    T. Vartio and A. Vaheri, A gelatin binding 70.000 dalton glycoprotein synthesized distinctly from fibronectin by normal and malignant adherent cells, J. Biol. Chem. (in press).Google Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • Sergio Barlati
    • 1
  • Giuseppina De Petro
    • 1
    • 2
  • Tapio Vartio
    • 2
    • 3
  • Antti Vaheri
    • 2
  1. 1.Istituto di Genetica Biochimica ed Evoluzionistica, C.N.RPaviaItaly
  2. 2.Department of VirologyUniversity of HelsinkiHelsinkiFinland
  3. 3.Department of PathologyUniversity of HelsinkiHelsinkiFinland

Personalised recommendations