Antibodies to Chromosomal Non-Histone Proteins in Malignant Cells

  • Lubomir S. Hnilica
  • Warren N. Schmidt
  • Marta Stryjecka-Zimmer
  • David M. Duhl
  • Zainy Banjar
  • Robert C. Briggs
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 57)


The exceptional selectivity of the immune response makes the use of antibodies to chromosomal proteins attractive for studies on the structure and function of chromatin. It is believed that alterations of cellular phenotype during differentiation and carcinogenesis are accompanied by changes in the composition of nuclear proteins, some of which may serve gene regulatory functions. Development of highly specific antisera would not only facilitate the detection, isolation and characterization of such regulatory proteins, but may also provide additional tools for the immunodetection of cancer. Isolated nuclear nonhistone proteins, 1−3nucleolar fractionsintact or dehistonized chromatins7−10 were all used as immunogens to elicit antisera which exhibited various degrees of specificity. Some antisera recognized antigens which changed during differentiation and development 7,11 or carcinogenesis.9,12 A considerable cell specificity was demonstrated for antisera to dehistonized chromatin preparations.2,7−14


Complement Fixation Chromosomal Protein Nitrocellulose Sheet Nonhistone Protein Antiserum Dilution 
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Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • Lubomir S. Hnilica
    • 1
    • 2
  • Warren N. Schmidt
    • 1
    • 2
  • Marta Stryjecka-Zimmer
    • 1
    • 2
  • David M. Duhl
    • 1
    • 2
  • Zainy Banjar
    • 1
    • 2
  • Robert C. Briggs
    • 1
    • 2
  1. 1.Department of Biochemistry and the A.B. Hancock, Jr. Memorial LaboratoryVanderbilt University Cancer CenterNashvilleUSA
  2. 2.Vanderbilt University School of MedicineNashvilleUSA

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