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Poly (A)-Polymerase Levels in Lymphoid Cells from Acute and Chronic Lymphocytic Leukemias

  • C. M. Tsiapalis
  • C. Ioannides
  • T. Trangas
  • N. Courtis
  • G. A. Pangalis
  • H. V. Cosmides
  • M. Papamichael
Part of the NATO Advanced Science Institutes Series book series (NSSA, volume 57)

Abstract

Poly (A) -polymerase is an enzyme found in a variety of eukaryotic cells and there is evidence that polyadenylation of HnRNA and mRNA is an early post-transcriptional process presumably mediated by poly (A)-polymerase. It has also been shown that the population of polyadenylated RNA molecules is constantly higher in the poorly differentiated and slowly growing human acute leukemic blast cells than normal and PHA-stimulated lymphocytes (Torelli, U and Torelli, G. Nature New Biol. 1973, 244,134). In the present investigation we have shown that the poorly differentiated human acute leukemic blast cells also have higher levels of poly (A) polymerase activity than the better differentiated chronic leukemic lymphocytes. Measurements of poly (A)-polymerase with poly (A)n and oligo (A)10 as initiators revealed differences in the enzyme activity with a level of significance 99%. The mean poly (A)-polymerase unit per mg of soluble protein in 10 CLL cases was 3.85, while in 10 ALL cases was 19.42.

Keywords

Acute Lymphocytic Leukemia Chronic Lymphocytic Leukemia Lymphoid Cell Polymerase Activity Cell Chronic Lymphocytic Leukemia 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • C. M. Tsiapalis
    • 1
  • C. Ioannides
    • 1
  • T. Trangas
    • 1
  • N. Courtis
    • 1
  • G. A. Pangalis
    • 2
  • H. V. Cosmides
    • 3
  • M. Papamichael
    • 1
  1. 1.Depts of Biochemistry and Immunology, Papanikolaou Research CenterUniversity of AthensGreece
  2. 2.First Dept. of Internal MedicineUniversity of AthensGreece
  3. 3.Second Dept. of PediatricsUniversity of AthensGreece

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