Androgenic Control of Gene Expression in Rat Ventral Prostate
Spectacular advances in recombinant DNA technology have enabled us to begin to analyze the structure and expression of many eukaryotic genes, including those whose expression is responsive to androgenic steroids. Such studies are essential if we are to understand how steroids influence gene expression because, although we know a great deal about steroid-receptor interactions in the cytoplasm, we know very little about events that take place in the cell nucleus. All classes of steroids appear to stimulate gene expression primarily by stimulating rates of mRNA production (Higgins and Gehring, 1978). It has also been shown, using both biochemical and genetic criteria, that hormone-receptor complexes bind to DNA and that this binding is related to a biological response (Yamamoto et al., 1974; Gronemeyer and Pongs, 1980). In view of this, it is generally postulated that hormone-receptor complexes bind directly to responsive genes, thereby stimulating their rate of transcription. The isolation of such genes is an essential prerequisite for testing this hypothesis. Furthermore, it is hoped that by analyzing the structure and organization of hormone responsive genes we will begin to understand what features are necessary for hormone responsiveness and what distinguishes such genes that give rise to tissue-specific gene expression.
KeywordsMouse Mammary Tumour Virus Ventral Prostate Recombinant Phage Primary Translation Product
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