Advertisement

Aspects of the Cellular Uptake and Retention of Hematoporphyrin Derivative and Their Correlation with the Biological Response to PRT in Vitro

  • B. W. Henderson
  • D. A. Bellnier
  • B. Ziring
  • T. J. Dougherty
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 160)

Abstract

The photosensitizer hematoporphyrin derivative (HPD) is known to be retained selectively in neoplastic tissues, which is the basis for its current use in the diagnosis of early neoplastic lesions, as well as in photoradiation therapy of a wide range of different tumors (1). The reason for porphyrin retention in tumors is not yet known. Porphyrin trapping in the tumor environment due to leaky vascularization and/or poor lymphatic drainage has been suggested as the mechanism (2). However, in view of the selective retention of HPD in very small CIS lesions (3,4), this explanation is not entirely satisfactory. Efforts to detect a special affinity for HPD to malignant cells in vitro have yielded controversial results (5–7). Uncertainty also prevails over the cellular target(s) of photoradiatfon injury. Damage to the cell membrane (8) as well as nuclear damage (9–11) have been reported.

Keywords

Cellular Uptake Hematoporphyrin Derivative Murine Leukemia L1210 Cell Photoradiation Therapy Poor Lymphatic Drainage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    T.J. Dougherty, K.R. Weishaupt and D.G. Boyle, Photorad-iation Therapy of Malignant Tumors, in: ‘Principles and Practice of Oncology’, V. DeVita, S. Hellman and S. Rosenberg, eds., J.B. Lippincott Publ. Co., New York (1981).Google Scholar
  2. 2.
    P.J. Bugelski, C.W. Porter and T.J. Dougherty, Autoradiographic Distribution of Hematoporphyrin Derivative in Normal and Tumor Tissue of the Mouse, Cancer Res., 41: 4606 (1981).PubMedGoogle Scholar
  3. 3.
    A.E. Profio and D.R. Doiron, Laser Fluorescence Bronchoscope for Localization of Occult Lung Tumors, Med. Phys.6:523 (1979).PubMedCrossRefGoogle Scholar
  4. 4.
    D.A. Cortese, J.H. Kinsey, L.B. Woolner, W.S. Payne, D.R. Sanderson and R.S. Fontana, Clinical Application of a new Endoscopic Technique for Detection of in situ Bronchial Carcinoma, Mayo Clin. Proc.54; 635 (1979).PubMedGoogle Scholar
  5. 5.
    B.T. Mossman, M.J. Gray, L. Silberman and R.L. Lipson, Identification of Neoplastic versus Normal Cells in Human Cervical Cell Culture, Obstet. Gynecol.43:635 (1974).PubMedGoogle Scholar
  6. 6.
    C.T. Chang and T.J. Dougherty, Photoradiation Therapy: Kinetics and Thermodynamics of Porphyrin Uptake and Loss in Normal and Malignant Cells in Culture, Radiat. Res.74:498 (1978).Google Scholar
  7. 7.
    T. Christensen, K. Feren, J. Moan and E. Pettersen, Photodynamic Effects of Hematoporphyrin Derivative on Synchronized and Asynchronous Cells of Different Origin, Br. J. Cancer, in press (1981).Google Scholar
  8. 8.
    D. Kessel, Transport and Binding of Hematoporphyrin Derivative and related Porphyrins by Murine Leukemia L1210 Cells, Cancer Res.41: 1318 (1981).PubMedGoogle Scholar
  9. 9.
    C.J. Gomer, K.R. Weishaupt and T.J. Dougherty, The Effects of Hematoporphyrin Derivative and Visible Red Light on V-79 Cells, Radiat. Res.74:586 (1978).Google Scholar
  10. 10.
    J. Moan and T. Christensen, Can Porphyrins be used in Diagnosis and Therapy of Cancer? Tumor Res. In Press.Google Scholar
  11. 11.
    D.A. Musser, N. Datta-Gupta and R.J. Fiel, Inhibition of DNA dependent RNA Synthesis by Porphyrin Photosensitizers, Biochem. Biophys. Res. Comm.97:918 (1980).PubMedCrossRefGoogle Scholar
  12. 12.
    S. Rockwell, In Vivo-in vitro Tumor Systems: New Models for Studying the Response of Tumors to Therapy, Lab. Animal Sci. 27: 831 (1977).Google Scholar
  13. 13.
    N.E. Fuseng and P.K.M. Worst, Mouse Epidermal Cell Cultures II. Isolation Characterization and Cultivation of Epidermal Cells from Perinatal Mouse Skin, Expt’l. Cell Res.93:443 (1975).CrossRefGoogle Scholar
  14. 14.
    C.J. Gomer and T.J. Dougherty, Determination of [3H]-and [14C]-Hematoporphyrin Derivative Distribution in Malignant and Normal Tissue, Cancer Res.39:146 (1979).PubMedGoogle Scholar
  15. 15.
    P.M. Ronai, The Elution of 51Cr from Labelled Leukocytes — A New Theory, Blood33:408 (1969).PubMedGoogle Scholar
  16. 16.
    C.J. Sanderson, The uptake and Retention of Chromium by cells, Transplantation21:256, (1976).Google Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • B. W. Henderson
    • 1
  • D. A. Bellnier
    • 1
  • B. Ziring
    • 1
  • T. J. Dougherty
    • 1
  1. 1.Roswell Park Memorial InstituteBuffaloUSA

Personalised recommendations