Aspects of the Cellular Uptake and Retention of Hematoporphyrin Derivative and Their Correlation with the Biological Response to PRT in Vitro
The photosensitizer hematoporphyrin derivative (HPD) is known to be retained selectively in neoplastic tissues, which is the basis for its current use in the diagnosis of early neoplastic lesions, as well as in photoradiation therapy of a wide range of different tumors (1). The reason for porphyrin retention in tumors is not yet known. Porphyrin trapping in the tumor environment due to leaky vascularization and/or poor lymphatic drainage has been suggested as the mechanism (2). However, in view of the selective retention of HPD in very small CIS lesions (3,4), this explanation is not entirely satisfactory. Efforts to detect a special affinity for HPD to malignant cells in vitro have yielded controversial results (5–7). Uncertainty also prevails over the cellular target(s) of photoradiatfon injury. Damage to the cell membrane (8) as well as nuclear damage (9–11) have been reported.
KeywordsCellular Uptake Hematoporphyrin Derivative Murine Leukemia L1210 Cell Photoradiation Therapy Poor Lymphatic Drainage
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