DNA Adduct Formation and Removal in N-Acetoxy-2-Acetylaminofluorene-Exposed Cultured Cells and in Organs from Rats Fed 2-Acetylaminofluorene

  • Miriam C. Poirier
  • Stuart H. Yuspa
  • B’Ann True
  • Brian A. Laishes
Part of the Basic Life Sciences book series


The development of carcinogen DNA-adduct antibodies has made possible a new approach to investigate carcinogen-DNA interactions (1). To quantitate adducts of a particular carcinogen, highly-avid rabbit antibodies have been employed to allow detection by radioimmunoassay (RIA) of one adduct in 10 5DNA bases (1). The studies described here employed the antiserum anti-guanosin-(8-yl)-acetylaminofluorene (anti-G-8-AAF) elicited against the nucleoside-adduct coupled covalently to bovine serum albumin and injected into rabbits (2). The antiserum is specific for the acetylated and deacetylated C-8 adducts of 2-acetylaminofluorene (2-AAF) with DNA (dG-8-AAF and dG-8-AF) (see Figure 1). It does not cross-react with the minor adduct, 3-deoxyguanison(N2-yl)-acetylaminofluorene (dG-N2-AAF) (see Figure 1), the carcinogen alone, or DNA (2,3). Since the C-8 adducts comprise the major proportion (80 to 90%) of adducts formed upon interaction of 2-AAF, or its activated derivative N-acetoxy-2-acetylaminofluorene (N-Ac-AAF) with DNA in vivo (3,4,5), the anti-G-8-AAF was considered appropriate for initial studies. The antibody has been utilized to distinguish between the acetylated and deacetylated C-8 adducts of 2-AM in DNA, and to quantitate the proportions of each in DNA extracted from either cultured cells exposed to N-Ac-AAF or from livers and kidneys of male rats fed 2-AAF.


High Pressure Liquid Chromatography Adduct Level Normal Human Fibroblast Cesium Chloride Retinyl Acetate 
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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • Miriam C. Poirier
    • 1
  • Stuart H. Yuspa
    • 1
  • B’Ann True
    • 2
  • Brian A. Laishes
    • 2
  1. 1.Laboratory of Experimental PathologyNational Cancer InstituteBethesdaUSA
  2. 2.McArdle Laboratory for Cancer ResearchUniversity of Wisconsin Medical SchoolMadisonUSA

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