Cell Specificity in DNA Damage and Repair
Many animal models for organ-specific neoplasia have been developed and used to investigate the pathogenesis of cancer. Studies dealing with morphologic aspects of this disease process have frequently concentrated on target cells. In contrast, most biochemical studies have employed whole organ homogenates even though tumors only develop from specific subpopulations of the target organ. For example, hepatocytes are the target cell for nitrosodiethylamine (DEN), 2-acetylaminofluorene, and dinitrotoluene, while sinusoidal lining cells represent the primary targets for vinyl chloride and 1,2-dimethylhydrazine (SDMH). Although hepatocytes comprise more than 90% of the liver’s mass, they account for only 60 to 70% of its cells. The nonparenchymal cells (NPC) account for the remaining 30 to 40% of the cells and 10 to 20% of the DNA. Thus, localization of carcinogen metabolism or DNA damage and repair within susceptible and nonsusceptible cell populations requires separation of cell types.
KeywordsVinyl Chloride Cell Replication Micrococcus Luteus Nonparenchymal Cell Nontarget Cell
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