Tissue-Specific Sister Chromatid Exchange Analyses in Mutagen-Carcinogen Exposed Animals

  • James W. Allen
  • Yousuf Sharief
  • Robert Langenbach
  • Michael D. Waters
Part of the Basic Life Sciences book series


The phenomenon of sister chromatid exchange (SCE) has been extensively reviewed (1–5). Sister chromatid exchanges are intrachromosomal events, wherein segments of DNA are reciprocally swapped between the chromatids. They are most easily studied with 5-bromodeoxyuridine (BrdU) dye methodology (6–8), which effectively differentiates the sister chromatids so that exchanges between them are detectable as staining discontinuities. Presumably, the exchange sites are at homologous loci and no inequality in the amount of translocated material results. Sister chromatid exchange is not known to alter cell viability or function; its spontaneous frequency and biological importance are uncertain. Yet, early autoradiography studies in cultured cells revealed elevated SCE frequencies as an effect of mutagen and carcinogen exposures (9). Consequently, with the development of more expeditious BrdU methodology, a course was set for accelerated studies of SCE formation in response to mutagen- or carcinogen-DNA interactions.


Sister Chromatid Sister Chromatid Exchange Ethyl Carbamate Phosphoramide Mustard Sister Chromatid Exchange Induction 
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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • James W. Allen
    • 1
  • Yousuf Sharief
    • 2
  • Robert Langenbach
    • 1
  • Michael D. Waters
    • 1
  1. 1.Genetic Toxicology Division, Health Effects Research LaboratoryU.S. Environmental Protection AgencyResearch Triangle ParkUSA
  2. 2.Northrop Services, Inc.Research Triangle ParkUSA

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