Species Differences in the Activation of Benzo[a]pyrene in the Tracheal Epithelium of Rats and Hamsters

  • Marc J. Mass
  • David G. Kaufman
Part of the Basic Life Sciences book series


Model systems have been developed to study bronchogenic carcinoma in laboratory animals in order to better understand the pathogenesis and etiology of this disease in humans (1). By extrapolating the characteristics of experimental models for respiratory carcinogenesis, some risk factors for human lung cancer may ultimately be elucidated.


High Pressure Liquid Chromatography Aryl Sulfatase Bronchogenic Carcinoma Intratracheal Instillation Tracheal Epithelium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Nettesheim, P., and R.A. Griesemer. 1978. Experimental models for studies of respiratory tract carcinogenesis. In: Pathogenesis and Therapy of Lung Cancer. C.C. Harris, ed. Marcel Dekker: New York. pp. 75–188.Google Scholar
  2. 2.
    Doll, R. 1978. An epidemiologic perspective of the biology of cancer. Cancer Rea. 38: 3573–3583.Google Scholar
  3. 3.
    Harris, C.C., H. Autrup, G.D. Stoner, S.K. Yang, J.C. Leutz, H.V. Gelboin, J.K. Selkirk, R.J. Conner, L.A. Barrett, R.T. Jones, E.M. McDowell, and B.F. Trump. 1977. Metabolism of benzo[a]pyrene and 7,12-dimethylbenz[a] anthracene in cultured human bronchus and pancreatic duct. Cancer Res. 37: 3349–3355.PubMedGoogle Scholar
  4. 4.
    Harris, C.C., H. Autrup, G.D. Stoner, B.F. Trump, E. Hillman, P.W. Schaefer, and A.M. Jeffrey. 1979. Metabolism of benzo[a]pyrene, N-nitrosodimethylamine, and N-nitrosopyrrolidine and identification of major carcinogen-DNA adducts formed in human esophagus. Cancer Res. 39: 4401–4406.PubMedGoogle Scholar
  5. 5.
    Autrup, H., C.C. Harris, B.F. Trump, and A.M. Jeffrey. 1978. Metabolism of benzo[a]pyrene and identification of major benzo[a]pyrene-DNA adducts in cultured human colon. Cancer Res. 38: 3689–3696.PubMedGoogle Scholar
  6. 6.
    Mass, M.J., N.T. Rodgers, and D.G. Kaufman. 1981. Benzo[a]pyrene metabolism in organ cultures of human endometrium. Chem.-Biol. Interact. 33: 195–205.Google Scholar
  7. 7.
    Harris, C.C., H. Autrup, R. Conner, L.A. Barrett, E.M. McDowell, and B.F. Trump. 1976. Interindividual variation in binding of benzo[a]pyrene to DNA in cultured human bronchi. Science 194: 1067–1069.PubMedCrossRefGoogle Scholar
  8. 8.
    Galofre, M.L., S.W. Payne, L.B. Woolner, 0.T. Clagett, and R.P. Gage. 1964. Pathologic classification and surgical treatment of bronchogenic carcinoma. Surg. Gynecol. Obstet. 119: 51–61.Google Scholar
  9. 9.
    Saffiotti, U., F. Cefis, and L.H. Kolb. 1968. A method for the experimental induction of bronchogenic carcinoma. Cancer Res. 28: 104–124.PubMedGoogle Scholar
  10. 10.
    Blair, W.H. 1974. Chemical induction of lung carcinomas in rats. In: Experimental Lung Cancer: Carcinogenesis and Bioassays. E. Karbe, and J.F. Parks, eds. Springer-Verlag: Berlin. pp. 199–206.CrossRefGoogle Scholar
  11. 11.
    Schreiber, H., D.H. Martin, and N. Pazmino. 1975. Species differences in the effect of benzo[a]pyrene-ferric oxide on the respiratory tract of rats and hamsters. Cancer Res. 35: 1654–1661.PubMedGoogle Scholar
  12. 12.
    Blair, W.H., N. Otero, and H. Rao. 1973. Development of lung neoplasms in rats treated with 7,12-dimethylbenz[a]anthracene. Proc. Am. Assoc. Cancer Res. 14: 497.Google Scholar
  13. 13.
    Schreiber, H., P. Nettesheim, and D.H. Martin. 1972. Rapid development of bronchiolo-alveolar squamous cell tumors in rats after intratracheal injection of 3-methylcholanthrene. J. Natl. Cancer Inst. 49: 541–546.Google Scholar
  14. 14.
    Mossman, B.T., and J.E. Craighead. 1978. Induction of neoplasms in hamster tracheal grafts with 3-methylcholanthrene-coated Lycra fibers. Cancer Res. 38: 3717–3722.PubMedGoogle Scholar
  15. 15.
    Smith, J.M., M.B. Sporn, D.A. Berkowitz, T. Kakefuda, E. Callan, and U. Saffiotti. 1971. Isolation of enzymatically active nuclei from epithelial cells of the trachea. Cancer Res. 31: 199–202.PubMedGoogle Scholar
  16. 16.
    Schaffner, W., and C. Weissman. 1973. A rapid, sensitive, and specific method for the determination of protein in dilute solution. Anal. Biochem. 56: 502–515.Google Scholar
  17. 17.
    Yang, S.K., H.V. Gelboin, H. Autrup, and C.C. Harris. 1977. Metabolic activation of benzo[a]pyrene and binding to DNA in cultured human bronchus. Cancer Res. 37: 1210–1215.PubMedGoogle Scholar
  18. 18.
    DePierre, J.W., M.S. Moron, K.A.M. Johannesen, and L. Ernster. 1975. A reliable, sensitive, and convenient radioactive assay for benzpyrene monooxygenase. Anal. Biochem. 63: 470–484.Google Scholar
  19. 19.
    Burke, M.D., H. Metabolism of derivatives.Vadi, B. Jernstrom, and S. Orrenius. 1977. benzo[a]pyrene and degradation of DNA-binding J. Biol. Chem. 18: 6424–6431.Google Scholar
  20. 20.
    Sims, P. 1967. homogenates.The metabolism of benzo[a]pyrene by rat liver Biochem. Pharmacol. 16: 613–618.Google Scholar
  21. 21.
    Yang, S.K., and H.V. Gelboin. 1976. Nonenzymatic reduction of benzo[a]pyrene diol-epoxides to trihydroxypentahydrobenzo[a]pyrenes by reduced nicotinamide adenine dinucleotide phosphate. Cancer Res. 36: 4185–4189.PubMedGoogle Scholar
  22. 22.
    Mass, M.J., and D.G. Kaufman. 1979. Benzo[a]pyrene quinone metabolism in tracheal organ cultures. Biochem. Biophys. Res. Comm. 89: 885–892.Google Scholar
  23. 23.
    Nettesheim, P., R.A. Griesemer, D.H. Martin, and J.E. Caton. 1977. Induction of preneoplastic and neoplastic lesions in grafted hamster tracheas continuously exposed to benzo[a]pyrene. Cancer Res. 37: 1272–1278.PubMedGoogle Scholar
  24. 24.
    Prough, R.A., V.W. Patrizi, R.T. Okita, B.S.S. Masters, and S.W. Jakobsson. 1979. Characteristics of benzo[a]pyrene metabolism by kidney, liver, and lung microsomal fractions from rodents and humans. Cancer Res. 39: 1199–1206.Google Scholar
  25. 25.
    Mass, M.J., and D.G. Kaufman. 1978. [3H]Benzo[a]pyrene metabolism in tracheal epithelial cell microsomes and tracheal organ cultures. Cancer Res. 38: 3861–3866.Google Scholar
  26. 26.
    Simberg, N., and P. Uotila. 1978. Stimulatory effect of cigarette smoke on the metabolism and covalent binding of benzo[a]pyrene in the trachea of the rat. Int. J. Cancer 22: 28–31.Google Scholar
  27. 27.
    Cohen, G.M., and B.P. Moore. 1976. Metabolism of benzo[a]pyrene by different portions of the respiratory tract. Biochem. Pharmacol. 25: 1623–1629.Google Scholar
  28. 28.
    Cohen, G.M., A.C. Marchok, P. Nettesheim, V.E. Steele, F. Nelson, S. Huang, and J.K. Selkirk. 1979. Comparative metabolism of benzo[a]pyrene in organ and cell cultures derived from rat tracheas. Cancer Res. 39: 1980–1984.PubMedGoogle Scholar
  29. 29.
    Moore, B.P., and G.M. Cohen. 1978. Metabolism of [3H]BP and its major metabolites to ethyl-acetate-soluble and water-soluble metabolites by cultured rodent trachea. Cancer Res. 38: 3066–3075.PubMedGoogle Scholar
  30. 30.
    Autrup, H., F.C. Wefald, A.M. Jeffrey, H. Tate, R.D. Schwartz, B.F. Trump, and C.C. Harris. 1980. Metabolism of benzo[a]pyrene by cultured tracheobronchial tissues from mice, rats, hamsters, bovines, and humans. Int. J. Cancer 25: 293–300.Google Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • Marc J. Mass
    • 1
  • David G. Kaufman
    • 1
  1. 1.Department of Pathology, School of MedicineUniversity of North CarolinaChapel HillUSA

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