Advertisement

Protein Phosphorylation and Chromatin Replication: Studies on the Interaction of Two Nuclear Proteins with DNA

  • Hans Stahl
  • Herbert König
  • Rolf Knippers

Summary

  1. 1

    Eukaryotic single strand specific DNA binding protein was phos-phorylated in vitro by a specific nuclear protein kinase. The untreated and phosphorylated protein have similar DNA binding properties. The phosphorylated binding protein, however, fails to stimulate DNA polymerase α.

     
  2. 2

    A growth dependent histone H1 specific protein kinase in vitro adds an average of three phosphate groups to one mole of isolated histone H1. Under appropriate ionic conditions, unmodified H1 binds cooperatively to DNA, while in vitro phosphorylated H1 binds distributively to DNA.

     

Keywords

Phosphate Group Protein Phosphorylation Sucrose Gradient Chromatin Protein Specific Protein Kinase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    L. J. Kleinsmith, in “The Cell Nucleus”, Vol. VI (“Chromatin”, Part C) (H. Busch, ed.) Acad. Press, New York, 1978.Google Scholar
  2. 2.
    M. Heil, Diploma-Thesis, Univ. Konstanz, 1981.Google Scholar
  3. 3.
    C. S. Teng, C. T. Teng and V. G. Allfrey, J. Biol. Chem. 246, 35–97, 1971.Google Scholar
  4. 4.
    W. Sons, H. J. Unsüld and R. Knippers, Eur. J. Biochem. 65, 263–269, 1976.PubMedCrossRefGoogle Scholar
  5. 5.
    R. Levy, S. Levi, S. Rosenberg and R. Simpson, Biochemistry, 12, 224, 1973.PubMedCrossRefGoogle Scholar
  6. 6.
    G. S. Stein, T. C. Spelsberg and L. J. Kleinsmith, Science, 183, 817–824, 1974.PubMedCrossRefGoogle Scholar
  7. 7.
    V. G. Allfrey, in: “Chromatin and Chromasome Structure” (H. J. Li and R. Eckhardt, eds.), Acad. Press, N. Y. 1977.Google Scholar
  8. 8.
    L. J. Kleinsmith and V. G. Allfrey, Biochim. Biophys. Acta 175, 123–135, 1969.PubMedGoogle Scholar
  9. 9.
    E. G. Krebs and J. A. Bearo, Ann. Rev. Biochem. 48, 923–959, 1979.PubMedCrossRefGoogle Scholar
  10. 10.
    J. Schlepper and R. Knippers, Eur. J. Biochem. 60, 209–220, 1975.PubMedCrossRefGoogle Scholar
  11. 11.
    H. Stahl and R. Knippers, Biochim. Biophys. Acta. 614, 71–80, 1980.PubMedGoogle Scholar
  12. 12.
    R. S. Lake and N. P. Salzman, Biochemistry 11, 4817–4823, 1972.PubMedCrossRefGoogle Scholar
  13. 13.
    R. A. Masarachia, B. E. Kemp and D. A. Walsh, J. Biol. Chem. 252, 7109–7117, 1977.Google Scholar
  14. 14.
    J. P. Whitlock, R. Augustine and H. Schulman, Nature 287, 74–76, 1980.PubMedCrossRefGoogle Scholar
  15. 15.
    B. Otto, M. Baynes and R. Knippers, Eur. J. Biochem. 73, 17–24, 1977.PubMedCrossRefGoogle Scholar
  16. 16.
    J. Szopa and H. Janska, Hoppe-Seyler’s Z. Physiol. Chemie 361, 1235–1241, 1980.CrossRefGoogle Scholar
  17. 17.
    Y. Hotta and H. Stern, Eur. J. Biochem. 95, 31–38, 1979.PubMedCrossRefGoogle Scholar
  18. 18.
    A. Richter, R. Knippers and B. Otto, FEBS Lett. 91, 293–296, 1978.PubMedCrossRefGoogle Scholar
  19. 19.
    P. Hohmann, R. A. Tobey and L. R. Gurley, J. Biol. Chem. 251, 3685–3692, 1976.PubMedGoogle Scholar
  20. 20.
    E. M. Bradbury, R. J. Inglis, H. R. Matthews and T. A. Laugan, Nature 249, 553–556, 1974.PubMedCrossRefGoogle Scholar
  21. 21.
    M. L. DePamphilis and P. M. Wassarman, Ann. Rev. Biochem. 49, 627–666, 1980.CrossRefGoogle Scholar
  22. 22.
    E. Fanning and I. Baumgartner, Virology 102, 1–12, 1980.PubMedCrossRefGoogle Scholar
  23. 23.
    G. M. T. Jones, S. C. Rail and R. D. Cole, J. Biol. Chem. 249, 2548–2553, 1974.PubMedGoogle Scholar
  24. 24.
    R. Knippers, B. Otto and R. Böhme, Nucl. Ac. Res. 5, 2113–2131, 1978.CrossRefGoogle Scholar
  25. 25.
    M. Renz and L. Day, Biochemistry 15, 3220–3228, 1976.PubMedCrossRefGoogle Scholar
  26. 26.
    J. Böhme, G. Keil and R. Knippers, Eur. J. Biochem. 78, 251–266, 1977.CrossRefGoogle Scholar
  27. 27.
    V. Jackson, A. Shires, N. Tanphaichitr and R. Chalkley, J. Mol. Biol. 104, 471–483, 1976.PubMedCrossRefGoogle Scholar
  28. 28.
    K. H. Klempnauer, E. Fanning, B. Otto and R. Knippers, J. Mol. Biol. 136, 359–374, 1980.PubMedCrossRefGoogle Scholar
  29. 29.
    A. Worcel, S. Hans and M. L. Wong, Cell 15, 969–977, 1978.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • Hans Stahl
    • 1
  • Herbert König
    • 1
  • Rolf Knippers
    • 1
  1. 1.Fakultät für BiologieUniversität KonstanzKonstanzGermany

Personalised recommendations