Protein Phosphorylation and Chromatin Replication: Studies on the Interaction of Two Nuclear Proteins with DNA

  • Hans Stahl
  • Herbert König
  • Rolf Knippers


  1. 1

    Eukaryotic single strand specific DNA binding protein was phos-phorylated in vitro by a specific nuclear protein kinase. The untreated and phosphorylated protein have similar DNA binding properties. The phosphorylated binding protein, however, fails to stimulate DNA polymerase α.

  2. 2

    A growth dependent histone H1 specific protein kinase in vitro adds an average of three phosphate groups to one mole of isolated histone H1. Under appropriate ionic conditions, unmodified H1 binds cooperatively to DNA, while in vitro phosphorylated H1 binds distributively to DNA.



Phosphate Group Protein Phosphorylation Sucrose Gradient Chromatin Protein Specific Protein Kinase 
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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • Hans Stahl
    • 1
  • Herbert König
    • 1
  • Rolf Knippers
    • 1
  1. 1.Fakultät für BiologieUniversität KonstanzKonstanzGermany

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