Adenovirus DNA Replication: Mechanism and Replication Proteins

  • Peter C. van der Vliet
  • Marijke M. Kwant
  • Bram G. M. van Bergen
  • Wim van Driel


The human adenoviruses, especially type 5 (Ad5) and type 2 (Ad2), can be easily cultivated in HeLa or KB cells. They have been a favorite subject for DNA replication studies in the eukaryotic cell during the last decade. The mature adenovirus genome isolated from virions consists of a linear duplex DNA molecule of about 35,000 base pairs. The DNA contains an inverted terminal repeat of 103 base pairs of which the first 50 nucleotides are very rich in A+T1,2. The inverted repeat is rather well conserved in the various adenoviruses and in particular base pairs 9–22 are identical in all human serotypes studied3.


Replication Fork Replication Complex Terminal Protein Daughter Strand Phosphonoacetic Acid 


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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • Peter C. van der Vliet
    • 1
  • Marijke M. Kwant
    • 1
  • Bram G. M. van Bergen
    • 1
  • Wim van Driel
    • 1
  1. 1.Laboratory for Physiological ChemistryUniversity of UtrechtUtrechtThe Netherlands

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