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Polyoma and Cell Chromatin Replication Studied in Mouse Cells which Exhibit Temperature-Sensitive DNA Synthesis because they are Sts or G1ts

  • Rose Sheinin
  • Richard Colwill
  • Peter R. Ganz

Abstract

Mammalian cells exhibiting temperature sensitive (ts) DNA synthesis have been exploited for two major purposes. The first was to elucidate specific biochemical reactions of cell cycle progression, the proteins which function therein and their genetic determinants. The second objective was to explore the mechanisms of interaction of polyoma virus DNA replication with those for the host genome; a specific goal being the identification of host proteins obligatorilylinked to viral DNA synthesis and integration into the cellular DNA. As a result of comparative biochemical and biophysical studies of cells which are dna ts /S ts or G1 ts , a modified model of chromatin replication has been formulated. We have demonstrated that the ts protein of the ts c1 dna ts /S ts or G1 ts , a modified model of chromatin of polyoma virus DNA. The gene product of the ts A1S9 dna ts /S ts mouse L-cell, which normally participates in conversion of newly-replicated single-stranded DNA of ≯ 5 × 106 daltons to chromosomal DNA of ≃ 109 molecular weight, plays a role in establishing or maintaining chromatin conformation essential for DNA replication and determines the novobiocin sensitivity of the cells.

Keywords

Chromatin Protein Temperature Inactivation Isoleucine Starvation84 Specific Biochemical Reaction Chromatin Replication 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • Rose Sheinin
    • 1
  • Richard Colwill
    • 1
  • Peter R. Ganz
    • 1
  1. 1.Department of Microbiology and Parasitology Faculty of MedicineUniversity of TorontoCanada

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