The Terminus of Chromosome Replication of E. coli Phenotypic Suppression of a dnaA Mutation by Plasmid Integration near terC

  • Jacqueline Louarn
  • Philippe Legrand
  • Josette Patte
  • Jean-Michel Louarn


The existence of a fixed termination region for chromosome replication in E. coli has been proposed1,2,3,4. In particular, we have shown that when a dnaAts mutation is phenotypically suppressed by an integrated plasmid (Integrative Suppression5), the replication forks initiated from the plasmid always meet in the rac (min 30)-man (min 35.5) region, irrespective of the plasmid insertion site on the chromosome. The terminus of replication, terC, was thus described primarily as a locus inhibiting replication fork movement in either direction. In addition, the termination step might be involved in regulatory operations of the cell cycle, as previously proposed6,7, but this possibility remains poorly documented. In the course of our previous analyses, as well as in other studies on integrative suppression by plasmid R100 derivatives8, integrative suppression by plasmid integration in a large region surrounding terC (grossly between 15 min and 45 min on the genetic map of Bachmann et al9 was never observed. If the restriction in the distribution of integration sites along the chromosome is related to terC functions, its analysis could constitute a way to investigate the role of the terminus.


Replication Fork Rich Medium Fusaric Acid Chromosome Replication Plasmid Integration 
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  1. 1.
    J. Louarn, J. Patte and J. M. Louarn, Evidence for a fixed termination site of chromosome replication in Escherichia coli K12, J. Mol. Biol. 115, 295–314, 1977.PubMedCrossRefGoogle Scholar
  2. 2.
    J. Louarn, J. Patte and J. M. Louarn, Map position of the replication terminus on the Escherichia coli chromosome. Mol. Gen. Genet. 172, 7–11, 1979.Google Scholar
  3. 3.
    P. Kuempel, S. Duerr and N. Seeley, The terminus of the chromosome in Escherichia coli inhibits replication forks. Proc. Natl. Acad. Sci. USA. 74, 3927–3931, 1977.PubMedCrossRefGoogle Scholar
  4. 4.
    P. Kuempel, S. Duerr and P. Maglothin, Chromosome replication in an Escherichia coli dnaA mutant integratively suppressed by prophage P2. J. Bacteriol. 134, 902–912, 1978.PubMedGoogle Scholar
  5. 5.
    Y. Nishimura, L. Caro, C. M. Berg and Y. Hirota, Chromosome replication in Escherichia coli. IV. Control of chromosome replication and cell division by an integrated episome. J. Mol. Biol. 55, 441–456, 1971.PubMedCrossRefGoogle Scholar
  6. 6.
    D. J. Clark, The regulation of DNA replication and cell division in E. coli B/r. Cold Spring Harbor Symp. Quant. Biol. 33, 823–838, 1968.PubMedCrossRefGoogle Scholar
  7. 7.
    N. C. Jones and W. D. Donachie, Chromosome replication, transcription and control of cell division in Escherichia coli. Nature New Biology 243, 100–103, 1973.PubMedCrossRefGoogle Scholar
  8. 8.
    A. Nishimura, Y. Nishimura and L. Caro, Isolation of Hfr strains from R+ and ColV2+ strains of Escherichia coli and derivation of an R1 lac factor. J. Bacteriol. 116, 1107–1112, 1973.PubMedGoogle Scholar
  9. 9.
    B. J. Bachmann and K. Brooks Low, Linkage map of Escherichia coli K12, Edition 6. Microbiol. Rev. 44, 1–56, 1980.PubMedGoogle Scholar
  10. 10.
    A. Campbell, D. Berg, D. Botstein, E. Lederberg, R. Novick, P. Starlinger and W. Szybalski, Nomenclature of transposable elements in procaryotes. DNA insertion elements, plasmids and episomes, 15-22, 1977.Google Scholar
  11. 11.
    N. Franklin, The N operon of lambda: Extent and regulation as observed in fusions to the tryptophan operon of Escherichia coli. In the bacteriophage lambda (A. D. Hershey, ed.). Cold Spring Harbor Press, New York, 621–638, 1971.Google Scholar
  12. 12.
    P. Legrand, J. P. Bouche and J. M. Louarn, Direction of deoxyribonucleic acid transfer and replication in a derivative of plasmic R100-1. J. Bacteriol. 140, 1105–1108, 1979.PubMedGoogle Scholar
  13. 13.
    Y. Hirota, A. Ryter and F. Jacob, Thermosens it ive mutants of E. coli affected in the process of DNA synthesis and cell division. Cold Spring Harbor Symp. Quant. Biol. 33, 677–693, 1968.PubMedCrossRefGoogle Scholar
  14. 14.
    D. Freifelder, A. Folkmanis and I. Krischner, Studies on Escherichia coli sex factors: Evidence that covalent circles exist within cells and the general problem of isolation of covalent circles. J. Bacteriol. 105, 722–727, 1971.PubMedGoogle Scholar
  15. 15.
    J. Miller, Experiments in molecular genetics. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory, 1972.Google Scholar
  16. 16.
    J. George, M. Castellazzi and G. Buttin, Prophage induction and cell division in E. coli. III. Mutations sfiA and sfiB restore division in tif and lon strains, and permit the expression of mutator properties of tif. Molee. Gen. Genet. 140, 309–332, 1975.Google Scholar
  17. 17.
    D. Kamp, R. Kahmann, D. Zipser, T. R. Broker and L. T. Chow, Inversion of the G DNA segment of phage Mu controls phage infectivity. Nature 271, 577–580, 1978.PubMedCrossRefGoogle Scholar
  18. 18.
    E. Ohtsubo, J. Feingold, H. Ohtsubo, D. Mickel and W. Bauer, Undirectional replication of three small plasmids derived from R factor R12 in Escherichia coli. Plasmid 1, 8–18, 1977.PubMedCrossRefGoogle Scholar
  19. 19.
    L. Silver, M. Chandler, E. Boy de la Tour and L. Caro, Origin and direction of replication of the drug resistance plasmid R100-1 and of a resistance transfer factor derivative in synchronized cultures. J. Bacteriol. 131, 929–942, 1977.PubMedGoogle Scholar
  20. 20.
    M. Chandler, L. Silver and L. Caro, Suppression of an Escherichia coli dnaA mutation by the integrated R factor R100-1. Ill Origin of chromosome replication during exponential growth. J. Bacteriol. 131, 421–430, 1977.PubMedGoogle Scholar
  21. 21.
    B. R. Bochner, H. C. Huang, G. L. Schieven and B. N. Ames, Positive selection for loss of tetracycline resistance. J. Bact. 143, 926–933, 1980.PubMedGoogle Scholar
  22. 22.
    R. H. Pritchard, Control of DNA replication in bacteria. In DNA synthesis: present and future: I. Molineux and M. Kohiyama ed., Plenum publ., New York, 1–26, 1978.Google Scholar
  23. 23.
    G. Kellenberger-Gujer, A. J. Podhajska and L. Caro, A cold sensitive dnaA mutant of E. coli which overinitiates chromosome replication at low temperature. Molec. Gen. Genet. 162, 9–22, 1978.PubMedCrossRefGoogle Scholar
  24. 24.
    K. G. Lark and C. A. Lark, recA+-dependent DNA replication in the absence of protein synthesis: characteristics of a dominant lethal mutation, dnaT, and requirement for recA + function. Cold Spring Harbor Symp. Quant. Biol. 43, 537–549, 1979.PubMedCrossRefGoogle Scholar
  25. 25.
    T. Kogoma, A novel Escherichia coli mutant capable of DNA replication in the absence of protein synthesis. J. Mol. Biol. 121, 55–69, 1978.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • Jacqueline Louarn
    • 1
  • Philippe Legrand
    • 1
  • Josette Patte
    • 1
  • Jean-Michel Louarn
    • 1
  1. 1.Centre de Recherche de Biochimie et de GénétiqueCellulaires du C.N.R.S.Toulouse CedexFrance

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