The Terminus of Chromosome Replication of E. coli Phenotypic Suppression of a dnaA Mutation by Plasmid Integration near terC
The existence of a fixed termination region for chromosome replication in E. coli has been proposed1,2,3,4. In particular, we have shown that when a dnaAts mutation is phenotypically suppressed by an integrated plasmid (Integrative Suppression5), the replication forks initiated from the plasmid always meet in the rac (min 30)-man (min 35.5) region, irrespective of the plasmid insertion site on the chromosome. The terminus of replication, terC, was thus described primarily as a locus inhibiting replication fork movement in either direction. In addition, the termination step might be involved in regulatory operations of the cell cycle, as previously proposed6,7, but this possibility remains poorly documented. In the course of our previous analyses, as well as in other studies on integrative suppression by plasmid R100 derivatives8, integrative suppression by plasmid integration in a large region surrounding terC (grossly between 15 min and 45 min on the genetic map of Bachmann et al9 was never observed. If the restriction in the distribution of integration sites along the chromosome is related to terC functions, its analysis could constitute a way to investigate the role of the terminus.
KeywordsReplication Fork Rich Medium Fusaric Acid Chromosome Replication Plasmid Integration
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© Plenum Press, New York 1983