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Enzymatic Mechanisms in DNA Replication: Initiation of Strands

  • Arthur Kornberg

Abstract

A model for the initiation of nascent (Okazaki) fragments in the discontinuous phase of replication is the conversion of ⌽X174 viral single-stranded (SS) DNA to the parental duplex replicative form (RF). The conversion in vitro requires at least eleven Escherichia coli proteins. Among these protein n’, a highly specific DNA-dependent ATPase (dATPase), guides proteins n, n”, i, dnaC dnaB and primase to the intragenic locus between structural genes F and G to assemble a mobile priming system, the primosome. Remarkably, the primosome, having served in the SS→RF reaction, is still physically and functionally present in nearly intact form following isolation by sucrose gradient centrifugation. During the next replicative stage (RF→RF), the conserved primosome facilitates cleavage, by the ⌽X-encoded gene A protein, of the viral strand, thereby initiating continuous elongation of this strand. When fully restored by the addition of protein i, the primosome repeatedly primes the discontinuous elongation of the complementary strand through several cycles of progeny RF production.

Keywords

Replication Fork Enzymatic Mechanism Replicative Form Duplex Form Priming Complex 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • Arthur Kornberg
    • 1
  1. 1.Department of BiochemistryStanford University School of MedicineStanfordUSA

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