Functionally Distinct Subpopulations of Human Monocytes: Receptors for F-Met-Leu-Phe are Expressed only on the Chemotactically Responsive Cells
In 1971 Horwitz and Garrett introduced a modification of the Boyden chamber method that made it possible to quantify chemotaxis of mononuclear phagocytes (1). The innovation was replacement of the 150 urn thick cellulose membrane separating cell and attractant chambers by a 10 urn thick polycarbonate membrane in which 5 urn diameter holes were randomly distributed. Macrophages migrated through these holes toward attractant and remained adherent to the attractant side of the membrane. One of the advantages of this method was that since all the migrated cells were in one optical plane, the number migrated could be readily counted. The authors showed that macrophages migrated toward E. coli filtrate, and that this was presumably not a chemokinetic effect, since migration was greatly reduced when equal concentrations of attractant were placed in cell and attractant wells. The method was also applied to blood monocytes (2) and many studies followed that showed decreases in the number of chemotaxin-responsive monocytes from patients with cancer and other diseases (3,4,5).
KeywordsHuman Monocyte Blood Monocyte Mononuclear Phagocyte Chemotactic Response Optical Plane
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