RES-Saturation with Accumulation of Hemoglobin-Haptoglobin Complexes in Plasma. A Method for Simultaneous Quantitation of Free Haptoglobin and Hemoglobin-Haptoglobin Complexes

  • Ivan Brandslund
  • Sven-Erik Svehag
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 155)

Abstract

The presence of visible amounts of hemoglobin in plasma is usually regarded as a certain sign of acute intravascular hemolysis. When hemoglobin (Hb) is released intravascularly it combines irreversibly with haptoglobin (Hp). One molecule Hp (MW 98–114 KD) binds up to 4 molecules of Hb (MW 16.2 KD) as 2 αβ units. These HbHp complexes have molecular weights ranging from 114,200 daltons when Hp is unsaturated to 162,800 daltons when Hp is fully saturated with four Hb molecules (assuming the genetic type of Hp with a molecular weight of 98,000 daltons). One g of Hp binds up to about 0.65 g Hb.

Keywords

Penicillin Fibrinogen Endocarditis 

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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Ivan Brandslund
    • 1
  • Sven-Erik Svehag
    • 2
  1. 1.Department of Clinical ChemistryOdense University HospitalOdense CDenmark
  2. 2.Institute of Medical MicrobiologyOdense UniversityOdense CDenmark

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