RES-Saturation with Accumulation of Hemoglobin-Haptoglobin Complexes in Plasma. A Method for Simultaneous Quantitation of Free Haptoglobin and Hemoglobin-Haptoglobin Complexes

  • Ivan Brandslund
  • Sven-Erik Svehag
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 155)


The presence of visible amounts of hemoglobin in plasma is usually regarded as a certain sign of acute intravascular hemolysis. When hemoglobin (Hb) is released intravascularly it combines irreversibly with haptoglobin (Hp). One molecule Hp (MW 98–114 KD) binds up to 4 molecules of Hb (MW 16.2 KD) as 2 αβ units. These HbHp complexes have molecular weights ranging from 114,200 daltons when Hp is unsaturated to 162,800 daltons when Hp is fully saturated with four Hb molecules (assuming the genetic type of Hp with a molecular weight of 98,000 daltons). One g of Hp binds up to about 0.65 g Hb.


Hemolytic Uremic Syndrome Fibrinogen Degradation Product Penicillin Treatment Simultaneous Quantitation Rocket Immunoelectrophoresis 
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  1. 1.
    Laurell, C. B., and Nyman, M., Blood 12:493, 1957.PubMedGoogle Scholar
  2. 2.
    Nyman, M., Scand. J. Clin. Lab. Invest. 11, suppl.39:1, 1959.PubMedGoogle Scholar
  3. 3.
    Müller-Eberhard, U., New England J. Med. 283:1091, 1970.CrossRefGoogle Scholar
  4. 4.
    Brandslund, I., and Hyltoft Petersen, P., in “Comptes Rendus du 4eColloque de Pont-a-Mousson de Biologie Prospective” (G. Siest and Galteau, M.-M., eds.), pp. 246–248, Masson, Paris, 1979.Google Scholar
  5. 5.
    Brandslund, I., Hyltoft Petersen, P., Brinkløv, M. M., Klint Anderson, P., and Parlev, E., Scand. J. Clin. Lab. Invest., in press.Google Scholar
  6. 6.
    Hersko, C., Cook, J. D., and Finch, C. A., J. Lab. Clin. Med. 80:624, 1972.Google Scholar
  7. 7.
    Liem, H. H., Biochem. Biophys. Acta 343:546, 1974.PubMedCrossRefGoogle Scholar
  8. 8.
    Wada, T., Ohara, H., Watanabe, K., Kinoshita, H., and Yachi, A., J. Reticuloendothelial Soc. 8:185, 1970.Google Scholar
  9. 9.
    Brandslund, I., and Hyltoft Petersen, P., Lancet II:633, 1979.CrossRefGoogle Scholar
  10. 10.
    Brandslund, I., and Hyltoft Petersen, P., Lancet I:103, 1980.CrossRefGoogle Scholar
  11. 11.
    Brandslund, I., Hyltoft Petersen, P., Strunge, P., Hole, P., and Worth, V., Haemostasis 9:193, 1980.PubMedGoogle Scholar
  12. 12.
    Brandslund, I., Siersted, H. C., Svehag, S.-E., and Teisner, B., J. Immunol. Methods 44:63, 1981.PubMedCrossRefGoogle Scholar
  13. 13.
    Saba, T. M., and Jaffe, E., Am. J. Med. 80:577, 1980.CrossRefGoogle Scholar
  14. 14.
    Walsh, R. T., and Barnhart, M. I., Thromb. Diath. Haemorrh. suppl. 36:83, 1969.PubMedGoogle Scholar
  15. 15.
    Brinkløv, M. M., Andersen, P. K., Nielsen, P. S., Brandslund, I., Hole, P., and Larsen, O., Uqeskr. Laeg 141:2606, 1979.Google Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Ivan Brandslund
    • 1
  • Sven-Erik Svehag
    • 2
  1. 1.Department of Clinical ChemistryOdense University HospitalOdense CDenmark
  2. 2.Institute of Medical MicrobiologyOdense UniversityOdense CDenmark

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