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Production of Macrophage Activating Factor by Cytolytic and Noncytolytic T Lymphocyte Clones

  • Anne Kelso
  • Andrew L. Glasebrook
  • K. Theodor Brunner
  • Osami Kanagawa
  • Howard D. Engers
  • H. Robson MacDonald
  • Jean-Charles Cerottini
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 155)

Abstract

Antigen-stimulated lymphoid populations release a soluble mediator(s), macrophage activating factor (MAF), which acts synergistically with nonactivating doses of lipopolysaccharide (LPS) to render macrophages nonspecifically tumoricidal (1–3). With the development of in vitro cloning techniques, it is now possible to examine directly the T lymphocytes which produce this and other lymphokines (4–9). Here we describe the phenotypes, antigen stimulation requirements and characteristics of lymphokine production by 72 clones generated against H-2, Mls, H-Y or Moloney leukemia virus (MoLV)-associated antigens.

Keywords

Spleen Cell Peritoneal Exudate Cell Mixed Lymphocyte Culture Lymphocyte Clone Lymphokine Production 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Anne Kelso
    • 1
    • 2
  • Andrew L. Glasebrook
    • 1
    • 2
  • K. Theodor Brunner
    • 1
    • 2
  • Osami Kanagawa
    • 1
    • 2
  • Howard D. Engers
    • 1
    • 2
  • H. Robson MacDonald
    • 1
    • 2
  • Jean-Charles Cerottini
    • 1
    • 2
  1. 1.Department of ImmunologySwiss Institute for Experimental Cancer ResearchEpalingesSwitzerland
  2. 2.The Ludwig Institute for Cancer ResearchEpalinges>Switzerland

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