Antigenic Markers on Human Monocytes and Macrophages
In an effort to examine heterogeneity within the human reticulo-endothelial system, we have developed a panel of monoclonal antibodies that define the expression of five distinct, nonpolymorphic antigens displayed on the surface of monocytes, macrophages and platelets (1,2). Antigen Mol is shared by peripheral blood monocytes, granulocytes, and a subset of Null cells. The expression of Mo2 and Mo3 is restricted to the monocyte-macrophage lineage. The density of Mo3, as distinguished from Mo2, is low on freshly isolated monocytes, but increases as a function of time in culture. Mo4 is borne by both monocytes and platelets, while Plt-1 is a platelet-specific antigen whose detection on monocytes reflects adsorption of platelets to monocyte surfaces. Mo1–4 are expressed by cultured macrophages derived from peripheral blood monocytes while only Mo1, 2, and 4 are borne by human peritoneal macrophages. Evidence for these conclusions will be presented and the significance of the Mo antigen series as differentiation markers within the monocyte-macrophage lineage will be discussed.
KeywordsAcute Lymphocytic Leukemia Peritoneal Macrophage Peripheral Blood Monocyte Myeloid Leukemia Cell Null Cell
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