Use of Glucan to Enhance Hemopoietic Recovery after Exposure to Cobalt-60 Irradiation
Glucan is a B-1,3 polyglucose isolated from the inner cell wall of the yeast Saccharomyces cerevisiae (1). Administration of glucan to rodents significantly enhances reticuloendothelial and immune responses (2–4) and represses tumor growth and experimental infections (5–7). Glucan also alters bone marrow and splenic hemopoietic proliferation and differentiation (8–13). Similar to glucan-induced immunomodulation, glucan-induced hemopoietic regulation depends on the route of glucan administration, the glucan dose administered, and the source of the glucan preparation (8,10,12). In general, following intravenous administration of currently available particulate glucan preparations in the dose range of 0.1 to 2.0 mg per mouse, the proliferation of bone marrow and splenic pluripotent stem cells (CFU-s), splenic macrophage and granulocyte-macrophage colony-forming cells (M-CFC, GM-CFC) and splenic erythroid colony and burst-forming cells (CFU-e, BFU-e) is stimulated in a direct, dose-dependent manner (12). The exact mechanisms through which glucan mediates its stimulatory effects on hemopoiesis are still largely unknown; however, both macrophages and T-lymphocytes have been reported to be involved (9,11,14).
KeywordsRadiation Control Splenic Macrophage Typing Skill Spleen Coloni Spleen Colony
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