Correlation between Two New Methods for the Isolation of Human Monocytes: Specific Reversible Agglutination with a Carbohydrate from Pokeweed and by use of the Lectin PA-4

  • M. J. Waxdal
  • C. Kieda
  • C. Murre
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 155)


Monocytes play essential roles in the activation of both T and B lymphocytes (1,2,3). To study the interactions of monocytes, as well as other cells, with the responding lymphocytes, methods of fractionating the different cell types are necessary. During our studies on cell surface carbohydrates and carbohydrate receptors on lymphoid cells, it became apparent that human monocytes strongly bound both a complex carbohydrate, and rather specifically bound the lectin Pa-4. These observations led to two new methods for the fractionation of human monocytes from peripheral blood.


Human Monocyte Peripheral Blood Leukocyte Cyanogen Bromide Cell Surface Carbohydrate Light Scatter Intensity 


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  1. 1.
    deVries, J. E., Caviles, A. P., Bont, W. S., and Mendelsohn, J., J. Immunol. 122:1099, 1979.Google Scholar
  2. 2.
    Mosier, D. E., Science 158:1573, 1967.PubMedCrossRefGoogle Scholar
  3. 3.
    Feldmann, M., J. Exp. Med. 135:1049, 1972.PubMedCrossRefGoogle Scholar
  4. 4.
    Kieda, C., and Waxdal, J. J., submitted for review.Google Scholar
  5. 5.
    Waxdal, M. J., Biochemistry 13:3671, 1974.PubMedCrossRefGoogle Scholar
  6. 6.
    Murre, C., Kieda, C., and Waxdal, M. J., submitted for review.Google Scholar
  7. 7.
    Tucker, S. B., Pierre, R. V., and Jordan, R. E., J. Immunol. Methods 14:267, 1977.PubMedCrossRefGoogle Scholar
  8. 8.
    Loken, M. R., and Herzenberg, L. A., Ann. N. Y. Acad. Sci. 254:163, 1975.PubMedCrossRefGoogle Scholar
  9. 9.
    Fowlkes, B. J., Waxdal, M. J., Sharrow, S. O., Thomas, C. A., Asofsky, R., and Mathieson, B. J., J. Immunol. 125:623, 1980.PubMedGoogle Scholar
  10. 10.
    Fauci, A. S., Pratt, K. R., and Whalen, G., J. Immunol. 117:2100, 1976.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • M. J. Waxdal
    • 1
  • C. Kieda
    • 1
  • C. Murre
    • 1
  1. 1.Laboratory of ImmunologyNational Institute of Allergy and Infectious Diseases National Institutes of HealthBethesdaUSA

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