Abstract
Monocytes play essential roles in the activation of both T and B lymphocytes (1,2,3). To study the interactions of monocytes, as well as other cells, with the responding lymphocytes, methods of fractionating the different cell types are necessary. During our studies on cell surface carbohydrates and carbohydrate receptors on lymphoid cells, it became apparent that human monocytes strongly bound both a complex carbohydrate, and rather specifically bound the lectin Pa-4. These observations led to two new methods for the fractionation of human monocytes from peripheral blood.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
deVries, J. E., Caviles, A. P., Bont, W. S., and Mendelsohn, J., J. Immunol. 122:1099, 1979.
Mosier, D. E., Science 158:1573, 1967.
Feldmann, M., J. Exp. Med. 135:1049, 1972.
Kieda, C., and Waxdal, J. J., submitted for review.
Waxdal, M. J., Biochemistry 13:3671, 1974.
Murre, C., Kieda, C., and Waxdal, M. J., submitted for review.
Tucker, S. B., Pierre, R. V., and Jordan, R. E., J. Immunol. Methods 14:267, 1977.
Loken, M. R., and Herzenberg, L. A., Ann. N. Y. Acad. Sci. 254:163, 1975.
Fowlkes, B. J., Waxdal, M. J., Sharrow, S. O., Thomas, C. A., Asofsky, R., and Mathieson, B. J., J. Immunol. 125:623, 1980.
Fauci, A. S., Pratt, K. R., and Whalen, G., J. Immunol. 117:2100, 1976.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1982 Plenum Press, New York
About this chapter
Cite this chapter
Waxdal, M.J., Kieda, C., Murre, C. (1982). Correlation between Two New Methods for the Isolation of Human Monocytes: Specific Reversible Agglutination with a Carbohydrate from Pokeweed and by use of the Lectin PA-4. In: Normann, S.J., Sorkin, E. (eds) Macrophages and Natural Killer Cells. Advances in Experimental Medicine and Biology, vol 155. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4394-3_23
Download citation
DOI: https://doi.org/10.1007/978-1-4684-4394-3_23
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-4396-7
Online ISBN: 978-1-4684-4394-3
eBook Packages: Springer Book Archive