Separation and Characterization of Guinea Pig Monocyte Subsets using Counter-Flow Centrifugation Elutriation (CCE)

  • S. J. Noga
  • S. J. Normann
  • R. Weiner
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 155)


Counter-flow centrifugation elutriation (CCE) has been used to separate human monocytes into two populations that differ in size, Fc receptors (FcR), antibody-dependent cellular cytotoxicity (ADCC), and native or acquired tumoricidal activity (1–4). To determine whether a similar monocyte heterogeneity exists in species other than man, we examined guinea pig (GP) monocytes. Unlike mouse and rat, GP peripheral blood has a white cell composition similar to humans (5). Our studies suggest that three distinct GP monocyte fractions can be isolated by CCE that differ in adherence, phagocytosis, and non-specific esterase activity as well as modal volume (MV), FcR’s, and acid phosphatase activity.


Modal Volume Acid Phosphatase Activity Phagocytic Ability Nylon Wool Intermediate Monocyte 
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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • S. J. Noga
    • 1
  • S. J. Normann
    • 1
  • R. Weiner
    • 1
  1. 1.Department of Pathology and Division of Medical OncologyUniversity of FloridaGainesvilleUSA

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