Separation and Characterization of Guinea Pig Monocyte Subsets using Counter-Flow Centrifugation Elutriation (CCE)
Counter-flow centrifugation elutriation (CCE) has been used to separate human monocytes into two populations that differ in size, Fc receptors (FcR), antibody-dependent cellular cytotoxicity (ADCC), and native or acquired tumoricidal activity (1–4). To determine whether a similar monocyte heterogeneity exists in species other than man, we examined guinea pig (GP) monocytes. Unlike mouse and rat, GP peripheral blood has a white cell composition similar to humans (5). Our studies suggest that three distinct GP monocyte fractions can be isolated by CCE that differ in adherence, phagocytosis, and non-specific esterase activity as well as modal volume (MV), FcR’s, and acid phosphatase activity.
KeywordsModal Volume Acid Phosphatase Activity Phagocytic Ability Nylon Wool Intermediate Monocyte
Unable to display preview. Download preview PDF.
- 4.Normann, S., and Weiner, R., in: “Heterogeneity of Mononuclear Phagocytes” (O. Förster and M. Landy, ed.), pp. 496–503, Academic Press, New York, 1981.Google Scholar
- 5.P. Altman, D. Katz, ed., in: “Inbred and Genetically Defined Strains of Laboratory Animals”, Part II, pp. 534–535, FASEB, Maryland, 1979.Google Scholar
- 7.Morrison, R., and Cutler, J., J. Reticuloendothelial Soc. 29:23, 1981.Google Scholar