Inhibition of Complement Dependent Phagocytosis by Normal Human Monocytes Following Preincubation with Immune Complexes
Interaction between immune complexes and cells of the monocyte/ macrophage lineage is important not only for the normal removal of immune complexes by the reticuloendothelial system but also in relation to defects found in putative immune complex diseases. Previous studies (1,2,3) with soluble complexes or immunoglobulin G aggregates have shown inhibition of Fc dependent monocyte or macrophage function, using relatively high concentrations of complexes. These studies used experimental designs in which the mononuclear phagocytes that had been preincubated with complexes did not encounter plasma, since preopsonised targets (usually erythrocytes) were used. In the course of studying phagocyte function in patients with a variety of autoallergic diseases, it was noted that patients with active immune complex disease (eg. systemic lupus erythematosis) had a defect of phagocytosis when tested using Candida guilliermondii targets in normal or autologous plasma (4). The effect of preincubating normal human monocytes with soluble heat-aggregated human IgG (AHG) or BSA anti BSA complexes was therefore investigated. Further studies were also performed using plasma from patients with immune complex diseases.
KeywordsImmune Complex Autologous Plasma Candida Guilliermondii Immune Complex Disease Mixed Essential Cryoglobulinemia
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- 4.Pinching, A. J., unpublished observations.Google Scholar