A Membrane Lectin on Macrophages: Localization and Demonstration of Receptor Recycling
It has been observed repeatedly that erythrocytes after neur-aminidase-treatment do not stay in circulation but adhere to Kupffer cells and thus are rapidly trapped in the liver (1). Spontaneous binding of neuraminidase-treated erythrocytes to Kupffer cells also occurs under serum free conditions in vitro (2,3). We have recently presented evidence that in vitro as well as in vivo cell contacts are due to a membrane lectin on Kupffer cells binding terminal Dgalactosyl residues exposed on desialylated erythrocytes (3–6). We now have localized the lectin on the macrophage cell surface. In studying the function of the receptor we have found that the lectin mediates endocytosis of particulate ligands followed by recycling of the receptor to the cell surface. A preliminary report of these findings has been published elsewhere (7).
KeywordsTrypsin Galactose Collagenase Cycloheximide Erythro
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