Differential Sensitivity to Certain DNA-Damaging Treatments, Including 8-Methoxypsoralen Plus 340 nm Ultraviolet Irradiation, Between K-12 and B/r Strains of Escherichia Coli

  • B. A. Bridges
  • A. von Wright
  • M. Stannard
  • E. Moustacchi
Part of the Basic Life Sciences book series (volume 15)


For want of information to the contrary, it is usually assumed that when a molecule of a mutagen is near to a molecule of DNA, reaction will occur with a probability that is essentially the same whether or not the DNA is in a bacterial or human chromosome, whether or not it is complexed with proteins, and whatever its degree of superhelicity. A moment’s thought suggests that this assumption is unlikely to be strictly true although it would be a bold man who would predict to what extent chromosomal structure might influence the probability of interaction with mutagens. The present paper records a simple bacterial model where the simplest interpretation of the data suggests that the structure rather than sequence of the DNA seems to affect the probability of interaction with intercalating agents such as 8-methoxypsoralen (8-MOP).


Differential Sensitivity Intercalate Agent Methane Sulphonate Methyl Methane Sulphonate Toluene Base Scintillation 
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  1. 1.
    Bridges, B. A. and Mottershead, R. P.: Inactivation of Escherichia coZi by near ultraviolet light and 8-methoxypsoralen: different responses of strains B/r and K-12. J. Bacteriol., 139: 454 (1979)PubMedGoogle Scholar
  2. 2.
    Clarke, C. H. and Wade, M. T.: Evidence that caffeine, 8methoxypsoralen and steroidal diamines are frameshift mutagens for E.coZi K-12. Mutation Res., 28: 123 (1975)PubMedCrossRefGoogle Scholar
  3. 3.
    Bridges, B. A. and Mottershead, R. P.: Frameshift mutagenesis in bacteria by 8-methoxypsoralen (methoxsalen) in the dark. Mutation Res., 44: 305 (1977)PubMedCrossRefGoogle Scholar
  4. 4.
    Howard-Flanders, P., Theriot, L. and Stedeford, J. B.: Some properties of excision-defective recombination-deficient mutants of Escherichia coil, K-12. J. Bacteriol., 97: 1134 (1969)PubMedGoogle Scholar
  5. 5.
    Bridges, B. A. and Sedgwick, S. G.: Effect of photoreactivation on the filling of gaps in deoxyribonucleic acid synthesised after exposure of Escherichia coZi to ultraviolet light. J. Bacteriol., 117: 1077 (1974)PubMedGoogle Scholar
  6. 6.
    Boyle, J. M. and Symonds, N.: Radiation-sensitive mutants of T4D.I.T4y; a new radiation-sensitive mutant: effect of the mutation on radiation survival, growth and recombination. Mutation Res., 8: 431 (1969)PubMedCrossRefGoogle Scholar
  7. 7.
    Hubbard, S. A., Green, M. H. L., Bridges, B. A., Wain, A. J. and Bridges, J. W.: The fluctuation test with S9 and hepatocyte activation, in Short Term Tests for Carcinogens ( Report of the International Collaborative Programme) Plenum Press, New York (1981).Google Scholar
  8. 8.
    Toogood, S. M.: The role of metabolism in some short term tests for chemical carcinogens and mutagens, M. Phil. Thesis, University of York (1979)Google Scholar
  9. 9.
    Peters, J. and Jagger, J.: Inducible repair of near-UV radiation lethal damage in E.coZi. Nature, 289: 194 (1981)PubMedCrossRefGoogle Scholar
  10. 10.
    Davies, B. D. and Mingioli, E. S.: Mutants of Escherichia coZi requiring methionine or vitamin B12. J. Bacteriol., 60: 17 (1950)Google Scholar
  11. 11.
    Bridges, B. A., Mottershead, R. P. and Knowles, A.: Mutation induction and killing of Escherichia coZi by DNA adducts and crosslinks: a photobiological study with 8-methoxypsoralen. Chem.-Biol. Interactions, 27: 221 (1979)Google Scholar
  12. 12.
    Drlica, K. and Snyder, M.: Superhelical Escherichia coZi DNA; relaxation by coumermycin. J. Molec. Biol., 120: 145 (1978)PubMedCrossRefGoogle Scholar
  13. 13.
    Worcel, A. and Burgi, E.: On the structure of the folded chromosome of Escherichia coZi. J. Molec. Biol., 71: 127 (1972)PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • B. A. Bridges
    • 1
  • A. von Wright
    • 1
  • M. Stannard
    • 1
  • E. Moustacchi
    • 2
  1. 1.MRC Cell Mutation UnitUniversity of Sussex FalmerBrightonEngland
  2. 2.Institute CurieCentre UniversitaireOrsay-CedexFrance

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