Regeneration of Plants from Protoplasts of Solanum Mammosum L

  • P. M. Kumar
  • S. K. Ghose
  • S. Sen
  • S. K. Sen
Part of the Basic Life Sciences book series (BLSC, volume 22)

Abstract

The potentials of isolated plant protoplasts for fundamental and applied research studies of crop plants are being appreciated more than ever as discussed by many workers recently (1,2). Solanurn mammosum L. is an exotic Solanaceous species and is considered a promising source of solasodine for pharmaceutical industries (3). This communication reports on the success that we attained in isolation and regeneration of cultured cell protoplasts of this medicinal crop plant.

Keywords

Sucrose Agar Cellulase Macromolecule Adenine 

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References

  1. 1.
    Y.P.S. Bajaj, Protoplast isolation, culture and somatic hybridization, in: “Plant Cell, Tissue and Organ Culture,” Y.P.S. Bajaj and J. Reinert, eds., Springer-Verlag, Berlin (1977).Google Scholar
  2. 2.
    I.K. Vasil, M.R. Ahuja and V. Vasil, Plant tissue cultures in genetics and plant breeding, Adv. in Genet. 20:127–215 (1979).CrossRefGoogle Scholar
  3. 3.
    T. Telek, H. Delpin and E. Cabanillas, Solanum mammosum as a source of solasodine in lowland tropics, Eco. Bot. 31:120–128 (1977).CrossRefGoogle Scholar
  4. 4.
    R.U. Schenk and A.C. Hildebrandt, Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures, Can. J. Bot. 50:199–204 (1972).CrossRefGoogle Scholar
  5. 5.
    J.B. Power and E.C. Cocking, Isolation of leaf protoplasts. Macromolecule uptake and growth substance response, J. Exp. Bot. 21:64–70 (1970).CrossRefGoogle Scholar
  6. 6.
    P.J. Larkin, Purification and viability determinations of plant protoplasts, Planta 128:213–216 (1976).CrossRefGoogle Scholar
  7. 7.
    I. Potrykus, C.T. Harms and H. Lorz, Multiple drop array (MDA) technique for the large scale testing of culture media variations in hanging microdrop cultures of single cell system. I. The technique, Plant Sci. Lett. 8:(1979).Google Scholar
  8. 8.
    P.M. Kumar, K. Das, R.R. Sinha, P. Mukherjee and S.K. Sen, Interspecific somatic protoplast fusion products in cultivated jute species, in: “Proc. of Intl. Symp. on Plant Cell Culture in Crop Improvement,” K.L. Giles and S.K. Sen, eds., Plenum Press, New York (in press) (1982).Google Scholar
  9. 9.
    T. Nagata and I. Takebe, Cell wall regeneration in and cell division of isolated tobacco mesophyll protoplasts, Planta 92:301–308 (1970).CrossRefGoogle Scholar
  10. 10.
    T. Murashige and F. Skoog, A revised medium for rapid growth and bioassays with tobacco tissue culture, Physiol. Plant. 15:473–497 (1962).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • P. M. Kumar
    • 1
  • S. K. Ghose
    • 2
  • S. Sen
    • 2
  • S. K. Sen
    • 1
  1. 1.Programme in Genetical Research, Department of BotanyBose InstituteCalcuttaIndia
  2. 2.Department of Genetics and Plant BreedingB. C. K. V. V.KalyaniIndia

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