Isolation and Regeneration of the Mesophyll Protoplasts of Brassica Juncea cv. Prakash
The availability of techniques for isolation and fusion of protoplasts has raised the possibility of their use in overcoming the barriers of hybridization in plant species (1). To achieve this goal it is imperative to work out conditions for the isolation and culture of protoplasts from the parental species. Mesophyll protoplasts of B. Napus (3, 4) and B. Oleracea var. botrytis (Bhartendu Vatsya personal comm.). The work described here deals with the isolation and culture of mesophyll protoplasts of Brassica juncea cv. Prakash.
KeywordsParental Species Brassica Juncea Mesophyll Protoplast Grown Plant Protoplast Isolation
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- 5.J.B. Power, E.C. Cocking, A simple method for the isolation of very large numbers of leaf protoplasts by using mixtures of cellulase and pectinase, Biochem. J., III:33–37 (1969).Google Scholar
- 6.K. Ohyama, J.P. Nitsch, Flowering haploid plants obtained from protoplasts of tobacco leaves, Plant Cell Physiol., 13:229–236 (1972).Google Scholar
- 7.H. Binding, Regeneration of haploid and diploid plants from protoplasts of Petunia hybrida, Z. Pf lanzenphysiol., 74:324–356 (1974).Google Scholar
- 8.D.R. Sharma, Studies on hybridization of somatic cells of plant origin in vitro, (Ph.D. Thesis, Division of Genetics, I.A.R.I., New Delhi) (1975).Google Scholar