Isolation and Regeneration of the Mesophyll Protoplasts of Brassica Juncea cv. Prakash

  • R. K. Aggarwal
  • D. R. Sharma
  • H. O. Mehra
  • R. K. Singh
Part of the Basic Life Sciences book series (BLSC, volume 22)


The availability of techniques for isolation and fusion of protoplasts has raised the possibility of their use in overcoming the barriers of hybridization in plant species (1). To achieve this goal it is imperative to work out conditions for the isolation and culture of protoplasts from the parental species. Mesophyll protoplasts of B. Napus (3, 4) and B. Oleracea var. botrytis (Bhartendu Vatsya personal comm.). The work described here deals with the isolation and culture of mesophyll protoplasts of Brassica juncea cv. Prakash.


Parental Species Brassica Juncea Mesophyll Protoplast Grown Plant Protoplast Isolation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • R. K. Aggarwal
    • 1
  • D. R. Sharma
    • 1
  • H. O. Mehra
    • 1
  • R. K. Singh
    • 1
  1. 1.Department of GeneticsHaryana Agricultural UniversityHissarIndia

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