Factors Responsible for the Production of Subprotoplasts in Brassica Oleracea var. Capitata
The semi-autonomous cytoplasmic organelles have genomes of their own, which are responsible wholly or partially for some traits which a plant breeder might like to incorporate into the nuclear genome background of another species. Thus, at times it may be desirable to produce cytoplasmic hybrids (cybrids) containing the cytoplasm of both but nucleus of only one of the parents (1). Such a combination is attainable by any of the follwoing means: X-irradiation of the protoplasts of one of the parents leading to the degeneration of its nuclei in hybrid cells (2); directional and total elimination of chromosomes of one of the parents in proliferating hybrid cells (3); and fusion of nucleated protoplasts with anucleated protoplasts of the other.
KeywordsCytoplasmic Male Sterility Acridine Orange Enzyme Mixture Protoplast Isolation Indian Agricultural Research Institute
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