Recovery of Virus Free Plantlets of Cultivated Jute Species

  • K. Das
  • R. R. Sinha
  • S. K. Sen
Part of the Basic Life Sciences book series (BLSC, volume 22)


Meristem culture techniques have helped in the past to generate symptomless or virus free plants of species of over 35 genera (1). As there exists a difference between the rates of plant growth and virus multiplication, it has been possible to culture the young and fast growing tissue, i.e., the meristem of a plant under virus free conditions. Meristem culture techniques date back about three decades (2). These techniques were first used in the fifties in order to free plants of virus (3). Since then it has been used to produce virus-free plants of several crops, including cassava (4), potatoes (5), white clover (6), red clover (7, 8), and alfalfa (8). These techniques have also been adopted for rapid asexual propagation of orchids (9), pea (10) and other crops.


Naphthalene Acetic Acid Meristem Culture Cassava Mosaic Disease Virus Free Plant Meristematic Dome 


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  1. 1.
    F. Quak, Meristem culture and virus free plants, in: “Plant Cell, Tissue and Organ Culture,” J. Reinert and Y.P.S. Bajaj, eds., Springer-Verlag, Berlin (1977).Google Scholar
  2. 2.
    E.A. Ball, Development in sterile culture of stem tips and subjacent region of Tropaeolum majus L. and of Lupinus alba L., Am. J. Bot. 33:301–308 (1946).CrossRefGoogle Scholar
  3. 3.
    G. Morel and C. Martin, Guerison de Dahlias atteints d’une maladic a virus, C.R. Acad. Sci. 235:1324–1325 (1952).Google Scholar
  4. 4.
    K.K. Kartha and O.L. Gamborg, Elimination of cassava mosaic disease by meristem culture, Phytopathology 65:826–828 (1975).CrossRefGoogle Scholar
  5. 5.
    F.C. Mellor and R. Stace-Smith, Virus-free potatoes by tissue culture, in: “Plant Cell, Tissue and Organ Culture,” J. Reinert and Y.P.S. Bajaj, eds., Springer-Verlag, Berlin (1977).Google Scholar
  6. 6.
    O.W. Barnett, P.B. Gibson and A. Seo, A comparison of heat treatment and meristem tip culture for obtaining virus-free plants of Trifolium repens, Plant Dis. Rep. 59:834–837 (1975).Google Scholar
  7. 7.
    G.C. Phillips and G.B. Collins, Virus symptomfree plants of red clover using meristem culture, Crop Sci. 19:213–216 (1979).CrossRefGoogle Scholar
  8. 8.
    V.A. Cheyne and P.J. Dale, Shoot tip culture in forage legumes, Plant Sci. Lett. 19:303–309 (1980).CrossRefGoogle Scholar
  9. 9.
    T. Murashige, Plant propagation through tissue cultures, Ann. Rev. Plant Physiol. 25:135–166 (1974).CrossRefGoogle Scholar
  10. 10.
    K.K. Kartha, O.L. Gamborg, F. Constabel and J.P. Shyluk, Regeneration of cassava plants from apical meristems, Plant Sci. Lett. 2:107–113 (1974).CrossRefGoogle Scholar
  11. 11.
    T. Murashige and F. Skoog, A revised meidum for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plant. 15:473–497 (1962).CrossRefGoogle Scholar
  12. 12.
    K. Das, “Application of Plant Cell/Tissue Culture Technique in Cultivated Jute Species of India for Their Possible Use in Crop Husbandry,” Ph.D. Thesis, Calcutta University, India (1982).Google Scholar
  13. 13.
    K.K. Kartha, Meristem culture and cryopreservation — methods and application, in: “Plant Tissue Culture,” T.A. Thorpe, ed., Academic Press, New York (1981).Google Scholar
  14. 14.
    O.M. Stone, Factors affecting the growth of carnation plants from shoot apices, Ann. Appl. Bot. 52:199–209 (1963).CrossRefGoogle Scholar
  15. 15.
    K. Mori, Production of virus-free plants by means of meristem culture, Jap. Agri. Res. Quart. 6:1–7 (1971).Google Scholar
  16. 16.
    P.J. Dale, Meristem tip culture in Lolium multiflorum, J. Exp. Bot. 26:731–736 (1975).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • K. Das
    • 1
  • R. R. Sinha
    • 1
  • S. K. Sen
    • 1
  1. 1.Programme in Genetical Research, Botany DepartmentBose InstituteCalcuttaIndia

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