Morphogenesis in Organ, Tissue and Cell Cultures of Some Species of Brassica

  • S. Singh
  • S. Banu
  • L. K. Pareek
  • N. Chandra
Part of the Basic Life Sciences book series (BLSC, volume 22)


There has been a widespread interest in cell, tissue and organ culture techniques for clonal propagation (1), haploid production and breeding (2), and mutagenesis (3). In spite of the large amount of work being done in this field there are many limitations of the method. One of the most important limitations has been a lack of detailed knowledge regarding the precise conditions of culture and control of differentiation in cell and organ cultures of important crop plants. Different species of Brassica have attracted worldwide attention because of their economic importance as sources of oil and vegetables (4–10). The present report pertains to the control of differentiation in tissue cultures of two oil yielding species B. campestris var. DYS-1 (Rape), B. juncea var. Prakash (mustard) and two vegetable crop plants B. oleracea var. botrytis (cauliflower) and B. oleracea var. capitata (cabbage).


Somatic Embryogenesis Suspension Culture Anther Culture Brassica Juncea Hypocotyl Explants 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    T. Murashige, Clonal propagation through tissue culture, in: “Plant Tissue Culture and Its Biotechnological Application,” W. Barz, E. Reinhard and M. H. Zenk, eds., Springer-Verlag, Berlin (1977).Google Scholar
  2. 2.
    G. Wenzel, Recent advances in applied tissue culture of potato rape and rye, in: “Plant Cell Culture Results and Perspectives,” F. Sala, B. Parisi, R. Cella and O. Ciferri, eds., Elsevier-North Holland, New York (1979).Google Scholar
  3. 3.
    P. Maliga, Isolation, characterization and utilization of mutant cell lines in higher plants, in: “Perspectives in Plant Cell and Tissue Culture,” I.K. Vasil, ed., International Review of Cytology (Suppl. 11A), London (1980).Google Scholar
  4. 4.
    T. Kameya and K. Hinata, Induction of haploid plants from pollen grains of Brassica, Jap. J. Breed. 20:82–87 (1970).Google Scholar
  5. 5.
    W.A. Keller, T. Rajhathy and J. Lacapra, In vitro production of plants from pollen in Brassica campestris, Can. J. Genet. Cytol. 17:655–666 (1975).Google Scholar
  6. 6.
    E. Thomas and G. Wenzel, Embryogenesis from microspores of Brassica napus, Z. Pflanzenzuchtg. 74:77–81 (1975).Google Scholar
  7. 7.
    L. George and P.S. Rao, In vitro regeneration of mustard plants (Brassica juncea var. Rai 5) on cotyledon expiants from nonirradiated, irradiated and mutagen treated seed, Ann. Bot. 46:107–112 (1980).Google Scholar
  8. 8.
    L.K. Pareek and N. Chandra, Somatic embryogenesis in leaf callus from cauliflower (Brassica oleracea var. botrytis), Plant Sci. Lett. 11:311–316 (1978).CrossRefGoogle Scholar
  9. 9.
    L.K. Pareek and N. Chandra, Induced regeneration in vegetative and flowering incernodal segments and ovary expiants of Brassica juncea coss., Indian J. Exp. Biol. 19:874–875 (1981).Google Scholar
  10. 10.
    S. Singh, S. Banu, L.K. Pareek and N. Chandra, Induced organ differentiation in hypocotyl segments, callus and suspension cultures of rape (Brassica campestris L.), Indian J. Exp. Biol. 19:658–660 (1981).Google Scholar
  11. 11.
    Y.P.S. Bajaj and P. Nietsch, In vitro propagation of red cabbage (Brassica oleracea L. var. capitata), J. Exp. Bot. 26:883–890 (1975).CrossRefGoogle Scholar
  12. 12.
    W.A. Keller and K.C. Armstrong, Embryogenesis and plant regeneration in Brassica napus anther culture, Can. J. Bot. 55:1383–1388 (1977).CrossRefGoogle Scholar
  13. 13.
    A.F. Mascarenhas, R.R. Hendre, A.L. Nadgir, D. Durga, M. Brave and V. Jagannathan, Differentiation in tissue culture of cabbage, Indian J. Exp. Biol. 16:122–125 (1978).Google Scholar
  14. 14.
    G. Wenzel, F. Hoffman and E. Thomas, Anther culture as a breeding tool in rape, Z. Pflanzenzuchtg. 78:149–155 (1977).Google Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • S. Singh
    • 1
  • S. Banu
    • 1
  • L. K. Pareek
    • 1
  • N. Chandra
    • 1
  1. 1.Department of BotanyUniversity of RajasthanJaipurIndia

Personalised recommendations