Tissue Culture in Diploid and Autotetraploid Strains of Metha (Trigonella Foenum-Graecum L.)
Trigonella foecum-graecum L. (fenugreek) a member of the Leguminosae is used as vegetable, forage and for preservative purposes. The seeds form an important source of diosgenin and yamogenin to be used in the steroid industry. In addition to the intact plant, cultured cells also yield secondary products. Callus formation and extraction of diosgenin or trigolline from static cultures has been demonstrated in Trigonella (1–6). Plant regeneration from leaf callus cultures have been reported in two species of Trigonella (7). However, no information is available on the callus growth and plant regeneration in different genotypes with various ploid levels. The present investigation was conducted to determine the response of five diploid and autotetraploid strains of Trigonella foenum-graecum L. for callus growth and plant regeneration from excised cotyledons, root and stem segments.
KeywordsCholesterol Sucrose Nicotinic Acid Kinetin Diosgenin
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