Amino Acid Analog Resistance in the Species of Soybean and Tobacco
Interest for isolating plant cell culture mutants has been increasing. Many cell lines with resistance to growth inhibition by amino acid analogs are now reported. Often they produce higher than normal amounts of the corresponding natural amino acids, and, hence, growth in specific amino acid analog is used as a selection tool (1). Several mechanisms can give rise to analog resistance, but most recovered variants appear to possess altered amino acid levels caused by changes in regulatory enzymes resulting in relaxed feed back control (1, 2). We attempted to select several amino acid analog resistant cell lines from plant suspenion cultures and a few of them derived from soybean and tobacco cell suspension are characterized. We present in this communication a brief report of our study.
KeywordsFree Amino Acid Resistant Line Resistant Cell Line Natural Amino Acid Total Free Amino Acid
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