Selection of Anther Derived Resistant Cell Lines of Solanum Khasianum to Culture Filtrate of Fusarium Species
Anther culture and its utilization has become a rapidly expanding field of research in recent years. In China, new varieties of tobacco, rice and wheat have been bred by means of this technique (1). Successes no doubt have attracted attention for the use of haploids in mutation studies for positive selection of resistant lines to herbicides, fungicides and pathogen toxins (2). It is known that the biochemical interaction between tissue cultures and pathogen toxins is usually identical to the whole plant response (3, 4). Mutant cell lines in haploids have been obtained in Nicotiana tabacum and Petunia hybrida for antibiotic resistance (5, 6). Albeit methods for producing haploids in Solanaceous plants have been established in a large number of species and interspecific hybrids, development of mutant lines against fungal pathogen has been reported in only Solanum tuberosum (7). Resistant maize plants against its parasite, Helminthosporium maydis (8) have been evolved.
KeywordsCallus Induction Culture Filtrate Anther Culture Fusaric Acid Fusarium Solani
Unable to display preview. Download preview PDF.
- 1.H. Han, Advances in anther culture investigations in China, in: “Proc. Symp. Plant Tissue Culture,” Science Press, Peking (1978).Google Scholar
- 2.E. Thomas, P.J. King and I. Potrykus, Improvement of crop plants via single cells in vitro — An assessment, Z. Pflanzenzuchtg. 82:14–16 (1979).Google Scholar
- 3.D.S. Ingram, Applications in plant pathology, in: “Plant Tissue and Cell Culture,” H.E. Street, ed., Blackwell Science Publ., Oxford (1977).Google Scholar
- 4.E.D. Earle, Phytotoxin studies with plant cells and protoplasts, in: “Frontiers of Plant Tissue Culture 1978,” T.A. Thorpe, ed., The International Association for Plant Tissue Culture, Alberta, Canada (1978).Google Scholar
- 6.P. Maliga, A. Sz-Breznovits and L. Marton, Streptomycin resistant plants from callus cultures of haploid tobacco, Nature (New Biol.) 144:29–30 (1973).Google Scholar
- 9.J.D. Mann, Production of solasodine for the pharmaceutical industry, in: “Advances in Agronomy,” N.C. Brady, ed. (1978).Google Scholar
- 11.C.C. Chu, C.C. Wang, C.S. Sun, C. Hsu, K.C. Yin and C.Y. Chu, Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources, Sci. Sinica 18:659–668 (1975).Google Scholar
- 14.R. Heitefuss, M.A. Stahmann and J.C Walker, Production of pectolytic enzymes and fusaric acid by Fusarium oxysporum F. Gonglutinass in relation to cabbage yellows, Phytopath. 50:367–370 (1960).Google Scholar
- 15.D.J. Heinz, Sugarcane improvement through induced mutations using vegetative propagules and cell culture techniques, in: “FAO/IAEA Panel Proc. Induced Mutations in Vegetatively Propagated Plants,” PL 501/5:53–59 (1973).Google Scholar
- 16.M. Krishnamurthi and J. Tlaskal, Fiji disease resistant Saccharum officinarum var. Pindar subclones from tissue culture, in: “Proc. Intl. Society Sugarcane Technologists,” XV:130–137 (1974).Google Scholar
- 17.J.P. Nitsch, Haploid plants from pollen, Z. Pflanzenzuchtg. 67:3–18 (1972).Google Scholar