In Vitro Isolation of Biochemical Mutants in Haploid Cell Cultures of Nicotiana Tabacum
Biochemical mutants in higher plants have several attractions. 1. They provide genetic markers for the selection of heterokaryons in studies with somatic hybridization and transformants in studies with DNA uptake (2). 2. They provide useful material for cell biological studies. 3. Some of the mutants like the pathotoxin resistant, amino-acid-analogue resistant (3) and herbicide resistant (4) could be of direct applied value.
KeywordsSomatic Hybridization Anther Culture Auxotrophic Mutant Ploidy Status Callus Piece
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