Expression of 5-Methyltryptophan Resistance in Regenerated Datura Innoxia Plants
Suspension cultures of Datura innoxia initiated from haploid plants were mutagenized with nitrosoguanidine and other compounds for 24 h, were rinsed into fresh medium and allowed to recover for four to seven days and then were plated in 46 M 5-methyltryptophan. After incubation for three to five months, growing colonies were placed into liquid medium and were tested for resistance to 5-methyltryptophan (Figure 1). In several experiments the untreated cells produced resistant colonies at the rate of 1.9 × 10−7 while nitrosoguanidine treatment increased this to 3.3 × 10−6.
KeywordsSuspension Culture Free Amino Acid Plant Cell Culture Haploid Plant Diploid Chromosome Number
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