Expression of 5-Methyltryptophan Resistance in Regenerated Datura Innoxia Plants

  • J. P. Ranch
  • J. M. Widholm
Part of the Basic Life Sciences book series (BLSC, volume 22)


Suspension cultures of Datura innoxia initiated from haploid plants were mutagenized with nitrosoguanidine and other compounds for 24 h, were rinsed into fresh medium and allowed to recover for four to seven days and then were plated in 46 M 5-methyltryptophan. After incubation for three to five months, growing colonies were placed into liquid medium and were tested for resistance to 5-methyltryptophan (Figure 1). In several experiments the untreated cells produced resistant colonies at the rate of 1.9 × 10−7 while nitrosoguanidine treatment increased this to 3.3 × 10−6.


Suspension Culture Free Amino Acid Plant Cell Culture Haploid Plant Diploid Chromosome Number 
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  1. 1.
    J.M. Widholm, The selection of agriculturally desirable traits with cultured plant cells, in: “Propagation of Higher Plants Through Tissue Culture — A Bridge Between Research and Application,” K.W. Hughes, R. Henke and M. Constantin, eds., Technical Information Center, U.S. Department of Energy, Washington, D.C. (1979).Google Scholar
  2. 2.
    T. Kameya, M.E. Horn and J.M. Widholm, Hybrid shoot formation from fused Daucus carota and D. capillifolius protoplasts, Z. Pflanzenphysiol. 104:459–466 (1981).Google Scholar
  3. 3.
    J. Widholm, Differential expression of amino acid biosynthetic control isoenzymes in plants and cultured cells, in: “Plant Cell Cultures: Results and Perspectives,” F. Sala, B. Parisi, R. Cella and O. Ciferri, eds., Elsevier/North-Holland Biomedical Press, Amsterdam (1980).Google Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • J. P. Ranch
    • 1
  • J. M. Widholm
    • 1
  1. 1.Department of AgronomyUniversity of IllinoisUrbanaUSA

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